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古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性
引用本文:韩彩霞,张丙昌,张元明,邰凤姣,张弛,邵华.古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性[J].中国沙漠,2016,36(4):1050-1055.
作者姓名:韩彩霞  张丙昌  张元明  邰凤姣  张弛  邵华
作者单位:1. 中国科学院新疆生态与地理研究所 干旱区生物地理与生物资源重点实验室, 新疆 乌鲁木齐 830011;2. 中国科学院新疆生态与地理研究所 荒漠与绿洲生态国家重点实验室, 新疆 乌鲁木齐 830011;3. 中国科学院大学, 北京 100049
基金项目:国家基础科学研究计划项目(2014CB954204);国家自然科学基金项目(U1203301,U1503103)
摘    要:为了解古尔班通古特沙漠南缘苔藓结皮中可培养真菌的多样性,运用稀释平板法和基于ITS-rDNA基因序列的系统发育分析,将得到的基因序列与NCBI数据库GenBank中的序列进行相似性比较并构建系统发育树。结果显示,从古尔班通古特沙漠南缘苔藓结皮中共分离得到24株菌落形态各异的真菌,分属于11属21种,绝大多数属于子囊菌亚门和半知菌亚门。其中,青霉属(Penicillium)和链格孢属(Alternaria)为优势菌群,茎点霉属(Phoma)、曲霉菌属(Aspergillus)、镰刀孢菌属(Fusarium)为常见属;有些种类的真菌具有耐热及特殊产酶活性。

关 键 词:古尔班通古特沙漠  苔藓结皮  可培养真菌  多样性指数  
收稿时间:2014-12-26
修稿时间:2015-04-08

Diversity of Culturable Fungi from the Moss Crusts Growing in the Southern Gurbantunggut Desert
Han Caixia,Zhang Bingchang,Zhang Yuanming,Tai Fengjiao,Zhang Chi,Shao Hua.Diversity of Culturable Fungi from the Moss Crusts Growing in the Southern Gurbantunggut Desert[J].Journal of Desert Research,2016,36(4):1050-1055.
Authors:Han Caixia  Zhang Bingchang  Zhang Yuanming  Tai Fengjiao  Zhang Chi  Shao Hua
Institution:1. Key Laboratory of Biogeography and Bioresource in Arid Land, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;2. State Key Laboratory of Desert and Oasis Ecology, Xinjiang Institute of Ecology and Geography, Chinese Academy of Sciences, Urumqi 830011, China;3. University of Chinese Academy of Sciences, Beijing 100049, China
Abstract:The objective of this study was to evaluate the diversity of culturable fungi isolated from the moss crusts growing in the Southern Gurbantunggut Desert. Dilution plate technique and ITS-rDNA sequence analysis were applied toobtain the sequences of 24 different colonies isolated from the moss crusts, which were subsequently submitted to GenBank and compared with those known sequences to build up the Phylogenetic tree. Results revealed that these strains were clustered into 3 phyla, 7 classes, 8 orders, 10 families, 12 genera, and the majority of them belong to Ascomycotina and Deuteromycetes. The dominant genera were Penicillium and Alternaria, the other genera included Phoma, Aspergillus, Fusarium, ect. We also isolated a thermotolerant fungi MF12 (KJ935021) and a fungi that produces special enzyme MF11 (KJ935022), implying the potential of utilizing the moss crust resource in the Southern Gurbantunggut Desert.
Keywords:Gurbantunggut Desert  moss crusts  culturable fungi  diversity index  
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