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Cytochrome P450 1A1 expression in cetacean skin biopsies from the Indian Ocean |
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| Authors: | Jauniaux Thierry Farnir Frédéric Fontaine Michaël Kiszka Jeremy Sarlet Michael Coignoul Freddy |
| Institution: | a Dept. of Pathology, Veterinary College, Sart Tilman B43, University of Liege, Belgium b Dept. of Biostatistics, Veterinary College, Sart Tilman B43, University of Liege, Belgium c Laboratoire Ecologie, Systématique et Evolution, Université Paris-Sud, Orsay, UMR8079, Orsay Cedex, F-91405, France d CNRS, UMR 8079, Orsay Cedex, F-91405, France e INRA, UMR CBGP, Campus international de Baillarguet, CS 30016, 34988 Montferrier-sur-Lez cedex, France f MARE Centre, Laboratory for Oceanology, University of Liège, B6c, Liège 4000, Belgium g Université de La Rochelle, LIENSS (Littoral, Environnement et Sociétés), UMR 6250, CNRS-Université de La Rochelle, 17071 La Rochelle, France h Direction de l’Environnement et du Développement Durable, Collectivité Départementale de Mayotte, BP 101 97600, Mayotte |
| Abstract: | The study describes cytochrome P450 1A1 (CYPA1) expression in the skin of different cetacean species (Megaptera novaeangliae, n = 15; Stenella attenuata, n = 7 and Stenella longirostris, n = 24) from the Mozambique Channel island of Mayotte. Immunohistochemical examination was performed with a monoclonal antibody against scup cytochrome CYPA1. The sex was determined using a molecular approach consisting in the genotyping sex-specific genes. CYPA1 was detected at the junction between epidermis and blubber on dolphins only, mostly in the endothelial cells. Similar observation was obtained in the dermis of one M. novaeangliae. Immunohistochemical slides were scored to evaluate the expression of the CYPA1 and a higher expression was observed in S. longirostris, suggesting a higher exposure to pollutants for this species. The difference of expression between sexes was not significant. |
| Keywords: | Cytochrome P450 Biopsy Skin Immunohistochemistry Mayotte |
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