Marine-derived filamentous fungi and their potential application for polycyclic aromatic hydrocarbon bioremediation |
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| Authors: | Passarini Michel R Z Rodrigues Marili V N da Silva Manuela Sette Lara D |
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| Institution: | a Universidade Estadual de Campinas, CPQBA, Rua Alexandre Cazellato, 999, CEP: 13140-000, Paulinia, SP, Brazil
b Fundação Oswaldo Cruz, Av. Brasil, 4365, CEP: 21040-900, Rio de Janeiro, RJ, Brazil |
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| Abstract: | Eight marine-derived fungi that were previously selected for their abilities to decolorize RBBR dye were subjected to pyrene and benzoa]pyrene degradation. The fungus Aspergillus sclerotiorum CBMAI 849 showed the best performance with regard to pyrene (99.7%) and benzoa]pyrene (76.6%) depletion after 8 and 16 days, respectively. Substantial amounts of benzoa]pyrene (>50.0%) depletion were also achieved by Mucor racemosus CBMAI 847. Therefore, these two fungal strains were subjected to metabolism evaluation using the HPLC-DAD-MS technique. The results showed that A. sclerotiorum CBMAI 849 and M. racemosus CBMAI 847 were able to metabolize pyrene to the corresponding pyrenylsulfate and were able to metabolize benzoa]pyrene to benzoa]pyrenylsulfate, suggesting that the mechanism of hydroxylation is mediated by a cytochrome P-450 monooxygenase, followed by conjugation with sulfate ions. Because these fungi were adapted to the marine environment, the strains that were used in the present study are considered to be attractive targets for the bioremediation of saline environments, such as ocean and marine sediments that are contaminated by PAHs. |
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| Keywords: | Pyrene Benzo[a]pyrene Pyrenylsulfate Benzo[a]pyrenylsulfate Marine-derived fungi Biodegradation |
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