Mercury-binding proteins in the slipper limpet,Crepidula fornicata,exposed to increased soluble mercury |
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Authors: | Florence L Harrison Kathleen Watness David A Nelson James E Miller Anthony Calabrese |
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Institution: | 1. Environmental Sciences Division, Lawrence Livermore National Laboratory, 94550, Livermore, California 2. Milford Laboratory, National Marine Fisheries Service, 06460, Milford, Connecticut
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Abstract: | Crepidula fornicata were held in a flow-through bioassay system and exposed to sand-filtered seawater to which no soluble mercury (control) was added or to which either 5, 25, or 50 μg 1?1 soluble Hg was added. At specific intervals during the 16-week experiment, a group of limpets was removed from each tank; one subgroup was exposed for 48 h to high concentrations of Hg, and another was analyzed for Hg-binding proteins by gelpermeation chromatography and spectrometry. Mortality from exposure to Hg in the 48-h acute toxicity tests was related to concentrations of Hg experienced both during the long-term exposure period and the 48-h exposure period. Chronic exposure to low levels of Hg resulted in increased amounts of total Hg in the whole body and in the low-molecular-weight Hg-binding proteins. No evidence was found for increased tolerance to Hg with preexposure. |
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