Genomic cloning and expression of vitellogenin gene from the self-fertilizing fish Rivulus marmoratus (Cyprinodontiformes, Rivulidae) |
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| Authors: | Kim Il-Chan Chang Sung Yeoul Williams Tim D Ja Kim Young Yoon Yong-Dal Lee Yong-Sung Park Eun-Ho Lee Jae-Seong |
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| Institution: | Department of Biochemistry, College of Medicine, Hanyang University, Seoul 133-791, Republic of Korea. |
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| Abstract: | We cloned the vitellogenin gene from the self-fertilizing fish Rivulus marmoratus, and sequenced 12,326 bp. The number of exons of R. marmoratus and rainbow trout vitellogenin genes were different, and also the splicing junctions are different throughout most of the exons and introns but the amino acid similarity of R. marmoratus vitellogenin gene to other species was rather high. In promoter region of R. marmoratus vitellogenin gene, there were several E2 binding sites and the estrogen response element (ERE). We discuss here the gene structure and expression of R. marmoratus vitellogenin gene. |
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