Paper II – Dirt,dates and DNA: OSL and radiocarbon chronologies of perennially frozen sediments in Siberia,and their implications for sedimentary ancient DNA studies |
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| Authors: | LEE J ARNOLD RICHARD G ROBERTS ROSS D E MACPHEE JAMES S HAILE FIONA BROCK PER MÖLLER DUANE G FROESE ALEXEI N TIKHONOV ALLAN R CHIVAS M THOMAS P GILBERT ESKE WILLERSLEV |
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| Institution: | 1. Centre for Archaeological Science, School of Earth and Environmental Sciences, University of Wollongong, Wollongong, NSW 2522, Australia, and Centro Nacional de Investigación sobre la Evolución Humana, CENIEH, 09002 Burgos, Spain;2. Centre for Archaeological Science, School of Earth and Environmental Sciences, University of Wollongong, Wollongong, NSW 2522, Australia;3. Division of Vertebrate Zoology, American Museum of Natural History, New York, NY 10024, USA;4. Centre for GeoGenetics, University of Copenhagen, DK‐2100 Copenhagen, Denmark;5. Oxford Radiocarbon Accelerator Unit, Research Laboratory for Archaeology and the History of Art, University of Oxford, Oxford OX1 3QY, UK;6. Department of Earth and Ecosystem Sciences, Division of Geology/Quaternary Sciences, Lund University, SE‐22362 Lund, Sweden;7. Department of Earth and Atmospheric Sciences, University of Alberta, Edmonton, Alberta T5M0M3, Canada;8. Laboratory of Mammals, Zoological Institute, Russian Academy of Sciences, 199034 St Petersburg, Russia;9. GeoQuEST Research Centre and Centre for Archaeological Science, School of Earth and Environmental Sciences, University of Wollongong, Wollongong, NSW 2522, Australia |
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| Abstract: | Arnold, L. J., Roberts, R. G., MacPhee, R. D. E., Haile, J. S., Brock, F., Möller, P., Froese, D. G., Tikhonov, A. N., Chivas, A. R., Gilbert, M. T. P. & Willerslev, E. 2010: Paper II – Dirt, dates and DNA: OSL and radiocarbon chronologies of perennially frozen sediments in Siberia, and their implications for sedimentary ancient DNA studies. Boreas, Vol. 40, pp. 417–445. 10.1111/j.1502‐3885.2010.00181.x. ISSN 0300‐9483 The sedimentary ancient DNA (sedaDNA) technique offers a potentially invaluable means of investigating species evolution and extinction dynamics in high‐latitude environments. An implicit assumption of the sedaDNA approach is that the extracted DNA is autochthonous with the host deposit and that it has not been physically transported from older source deposits or reworked within the sedimentary profile by postdepositional mixing. In this paper we investigate whether these fundamental conditions are upheld at seven perennially frozen wetland sites across the Taimyr Peninsula and coastal lowlands of north‐central Siberia. Optically stimulated luminescence (OSL) and radiocarbon (14C) dating are used to constrain the ages of both the inorganic and organic fractions of perennially frozen deposits from which sedaDNA of extinct and extant species have been recovered. OSL and 14C age/depth profiles, as well as single‐grain equivalent dose (De) distribution characteristics, are used to assess the stratigraphic integrity of these sedaDNA sequences by (i) identifying the presence of primary or reworked organic and inorganic material, and (ii) examining the types of depositional and postdepositional processes that have affected specific sedimentary facies. The results of this study demonstrate that even though DNA preservation and stratigraphic integrity are commonly superior in perennially frozen settings, this does not, in itself, guarantee the suitability of the sedaDNA approach. The combined OSL and 14C chronologies reveal that certain perennially frozen sites may be poorly suited for sedaDNA analysis, and that careful site selection is paramount to ensuring the accuracy of any sedaDNA study – particularly for ‘latest appearance date’ estimates of extinct taxa. |
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