| 根据mtDNA D-loop 序列分析东海银鲳群体遗传多样性 |
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| 引用本文： | 彭士明,施兆鸿,陈 超,侯俊利.根据mtDNA D-loop 序列分析东海银鲳群体遗传多样性[J].海洋科学,2010,34(2):28-32. |
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| 作者姓名： | 彭士明 施兆鸿 陈 超 侯俊利 |
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| 作者单位： | 1. 中国水产科学研究院,东海水产研究所,上海,200090
2. 中国水产科学研究院,黄海水产研究所,山东,青岛,266071 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费资助项目(东2008M14, 东2007Z02) |
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| 摘 要： | 根据线粒体D-loop序列对舟山群岛附近海域的银鲳(Pampus argenteus)群体(n=24)的遗传多样性进行了研究。通过PCR技术对线粒体D-loop序列进行扩增,获得大小约为500bp的扩增产物。PCR产物经纯化并进行序列测定后,得到了357bp的核苷酸片段。在24个个体中,共检测到14个变异位点,其中8个转换位点,5个颠换位点,1个转换与颠换同时存在的位点。运用MEGA软件计算出不同个体间的遗传距离,并根据其遗传距离构建了UPGMA和NJ系统树。DNASP软件计算出的单倍型多样性(h)、核苷酸多样性(π)及平均核苷酸差异数(k)分别为0.89、0.007与2.57。此外,岐点分布及中性检验显示,东海银鲳群体在历史上可能经历过种群扩张。研究结果表明,线粒体D-loop基因可用于银鲳群体内及群体间遗传多样性的分析。
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| 关 键 词： | 银鲳(Pampus argenteus) 东海 线粒体DNA D-loop 序列 |
| 收稿时间： | 2009/3/20 0:00:00 |
| 修稿时间： | 7/3/2009 12:00:00 AM |
Genetic diversity analysis of silver pomfret (Pampus argenteus) in the East China Sea based on mtDNA D-loop sequence |
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| PENG Shi-ming,SHI Zhao-hong,CHEN Chao and HOU Jun-li.Genetic diversity analysis of silver pomfret (Pampus argenteus) in the East China Sea based on mtDNA D-loop sequence[J].Marine Sciences,2010,34(2):28-32. |
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| Authors: | PENG Shi-ming SHI Zhao-hong CHEN Chao and HOU Jun-li |
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| Institution: | 1.East China Sea Fisheries Research Institute;Chinese Academy of Fishery Sciences;Shanghai 200090;China;2. Yellow Sea Fisheries Research Institute;Qingdao 266071;China |
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| Abstract: | Genetic diversity of silver pomfret (Pampus argenteus) in the East China Sea was investigated based on mtDNA D-loop sequences. The PCR technique was used to amplify the mtDNA D-loop in 24 individuals of silver pomfret collected from the East China Sea,near the coast waters of Zhoushan Islands. The PCR products were pu-rified and sequenced. The results showed that 357bp nucleotide sequences of partial D-loop gene were obtained (the primer and some of the marginal sequences were excluded). Among the 24 indivi... |
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| Keywords: | Pampus argenteus East China Sea mitochondrial DNA D-loop sequence |
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