| 紫贻贝(Mytilus edulis)与厚壳贻贝(M.coruscus)杂交的细胞学观察及子一代的早期生长比较 |
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| 引用本文： | 陈兴强,李荣华,王春琳,胡益鸣,程继生,王成,詹萍萍,母昌考,宋微微,龚小敏.紫贻贝(Mytilus edulis)与厚壳贻贝(M.coruscus)杂交的细胞学观察及子一代的早期生长比较[J].海洋与湖沼,2017,48(3):600-608. |
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| 作者姓名： | 陈兴强 李荣华 王春琳 胡益鸣 程继生 王成 詹萍萍 母昌考 宋微微 龚小敏 |
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| 作者单位： | 宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,宁波大学 应用海洋生物技术教育部重点实验室 宁波 315211;浙江海洋高效健康养殖协同创新中心 宁波 315211,象山鹤浦镇人民政府海洋与渔业办公室 象山 315733 |
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| 基金项目： | 浙江省贻贝产业服务团队科技特派员项目，2013.09-2017.12；浙江省重中之重学科开放基金项目，xkzsc1529号；宁波市科技富民项目，2015C10006号；王宽诚基金资助。 |
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| 摘 要： | 为了研究紫贻贝与厚壳贻贝种间杂交的核相变化,并评估其杂交优势,本实验以两种贻贝亲本为材料建立了4个交配组合,分别为:紫贻贝自交组EE(Mytilus edulis♀×M.edulis♂)、厚壳贻贝自交组CC(Mytilus coruscus♀×M.coruscus♂)、正交组EC(M.edulis♀×M.coruscus♂)和反交组CE(M.coruscus♀×M.edulis♂)。通过Hoechst 33258染色、荧光显微观察的方法,对4个交配组从受精、减数分裂到卵裂的核相变化过程进行了观察,并对孵化后幼虫到稚贝期的生长情况进行了记录。结果表明:正反交组的受精细胞学过程与自交组较为相似,但第二极体排出时间延长。对各实验组子一代早期的生长和存活研究表明:浮游阶段,杂交组幼虫生长优势不明显;附着变态后,正交组EC在壳长及壳高等生长指标的杂交优势表现明显,且正交组(EC)大于反交组(CE)。自交组的受精率、孵化率、成活率均高于杂交组。
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| 关 键 词： | 紫贻贝 厚壳贻贝 杂交 细胞学观察 生长比较 |
| 收稿时间： | 2016/12/16 0:00:00 |
| 修稿时间： | 2017/1/4 0:00:00 |
CYTOLOGICAL OBSERVATION ON NUCLEAR BEHAVIOR OF HYBRIDS BETWEEN MYTILUS EDULIS AND M. CORUSCUS AND COMPARISON IN EARLY-STAGE GROWTH |
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| CHEN Xing-Qiang,LI Rong-Hu,WANG Chun-Lin,HU Yi-Ming,CHENG Ji-Sheng,WANG Cheng,ZHAN Ping-Ping,MU Chang-Kao,SONG Wei-Wei and GONG Xiao-Min.CYTOLOGICAL OBSERVATION ON NUCLEAR BEHAVIOR OF HYBRIDS BETWEEN MYTILUS EDULIS AND M. CORUSCUS AND COMPARISON IN EARLY-STAGE GROWTH[J].Oceanologia Et Limnologia Sinica,2017,48(3):600-608. |
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| Authors: | CHEN Xing-Qiang LI Rong-Hu WANG Chun-Lin HU Yi-Ming CHENG Ji-Sheng WANG Cheng ZHAN Ping-Ping MU Chang-Kao SONG Wei-Wei and GONG Xiao-Min |
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| Institution: | Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China,Key Laboratory of Applied Marine Biotechnology, Ministry of Education, School of Marine Science, Ningbo University, Ningbo 315211, China;Collaborative Innovation Center for Zhejiang Marine High-efficiency and Healthy Aquaculture, Ningbo University, Ningbo 315211, China and Ocean and Fishery Office of People''s Government of Hepu, Xiangshan 315733, China |
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| Abstract: | |
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| Keywords: | Mytilus edulis Mytilus coruscus hybridization cytological observation growth comparison |
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