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虾池微藻定向培育及其对养殖环境因子的影响
引用本文:黄翔鹄,冯奕成,李长玲,刘楚吾.虾池微藻定向培育及其对养殖环境因子的影响[J].广东海洋大学学报,2005,25(6):25-31.
作者姓名:黄翔鹄  冯奕成  李长玲  刘楚吾
作者单位:1. 广东海洋大学水产学院,广东,湛江,524088
2. 东平镇人民政府,广东,阳东,529900
摘    要:采用微藻三级扩大培养技术,将在对虾养殖池选育的波吉卵囊藻Oocysits borgei引入凡纳滨对虾Litopenaeusvannamei高位养殖池,并检测养殖环境中微藻群落结构、水质因子、对虾抗病力相关因子、对虾生长情况,研究了以微藻生态调控为主的对虾防病技术。结果表明:养殖前、中、后期,波吉卵囊藻平均生物量占浮游植物总生物量分别为98.02%、78.89%和45.12%,成为虾池中的绝对优势种;作为优势种的持续时间长达77 d。波吉卵囊藻为主的微藻群落控制的水质较稳定,实验池氨氮和亚硝酸盐氮浓度较对照池低。对虾的血细胞数、溶菌(LSZ)活力、抗菌活力、超氧化物歧化酶(SOD)和酚氧化酶(PO)均显著高于对照池(P<0.05);血清蛋白含量差异不显著(P>0.05)。实验池对虾生长速度显著高于对照池(P<0.05)。可见,通过微藻的定向培育方法来优化虾池微藻群落结构,可改善养殖环境。

关 键 词:微藻  波吉卵囊藻  定向培育  微藻群落结构  凡纳滨对虾  生态防病
文章编号:1007-7995(2005)06-0025-07
修稿时间:2005年8月28日

Directive Breeding of Microalgae and Effects of It on Environment Factors in Prawn Pond
HUANG Xiang-hu,FENG Yi-cheng,LI Chang-ling,LIU Chu-wu.Directive Breeding of Microalgae and Effects of It on Environment Factors in Prawn Pond[J].Journal of Zhanjiang Ocean University,2005,25(6):25-31.
Authors:HUANG Xiang-hu  FENG Yi-cheng  LI Chang-ling  LIU Chu-wu
Abstract:Oocystis borgei,which was selected from the prawn culture pond and expanded after 3-grade cuture,was artificially introduced into the culture system of Litopenaeus vannamei.The structure of microalgae community,the water quality,the factors that relate to the anti-disease ability of prawn and the growth of prawn were inspected to study the technology of microalage-ecology control to prevent shrimp diseases.The result showed that the average biomass of Oocystis borgei in the early,middle and late period of prawn culture was 98.02%,78.89% and 45.12%,respectively of the total microalgae.As the dominant species,it lasted for about 77 days in the prawn pond.The water quality was more stability when the dominant species was mainly Oocystis borgei in it.Ammonia nitrogen and nitrite nitrogen in the experiment pond was lower than that of the control pond.The number of blood cell,the activity of phenoloxidase,superoxide dismutase(SOD),antibacterial enzyme and lysozyme were significantly higher(P<0.05) than the control pond.The content of blood serum protein was not significantly differentia(P>0.05).The cultural environment was improved via optimizing the structure of microalgae community by directive breeding microalgae.
Keywords:microalgae  Oocystis borgei  directive breeding  microalgae community structure  Litopenaeus vannamei  ecological disease preventing
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