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| 二阶导数线性扫描极谱法测定秋茄叶中总黄酮含量 | |
| 引用本文： | 范润珍,林树群,杨素萍,宋文东.二阶导数线性扫描极谱法测定秋茄叶中总黄酮含量[J].台湾海峡,2011,30(1):138-141. |
| 作者姓名： | 范润珍 林树群 杨素萍 宋文东 |
| 作者单位： | 1. 广东海洋大学理学院,广东,湛江,524025 2. 汕头市美宝制药有限公司,广东,汕头,515041 3. 河北北方学院基础农学部,河北,张家口,075000 |
| 基金项目： | 广东省自然科学基金资助项目，湛江市科技招标资助项目 |
| 摘 要： | 探讨了秋茄叶中黄酮类物质的极谱特性,并进行了含量测定.结果表明,在pH值为9.18的硼砂底液中,以芦丁作对照品,秋茄叶中黄酮类化合物在峰电位Ep为-1 642 mV（vs.SCE）处产生一灵敏的二阶导数极谱波,在芦丁含量不超过1.4 mg/dm3范围内,该极谱波的峰电流（Ip）与芦丁的量之间呈良好的线性关系,据此建立了红树秋茄叶中总黄酮含量的极谱定量测定方法.该方法的检出限为0.02 mg/dm3.用此法测得红树秋茄叶中总黄酮含量为2.04%,回收率为96.4%～100.3%,相对标准偏差为3.19%,此方法灵敏、准确、干扰少. |
| 关 键 词： | 海洋化学 秋茄 黄酮 二阶导数线性扫描极谱法 |
Determination of total flavones in Kandelia candel leaves by second derivative linear-sweep polarography |
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| FAN Run-zhen,LIN Shu-qun,YANG Su-ping,SONG Wen-dong.Determination of total flavones in Kandelia candel leaves by second derivative linear-sweep polarography[J].Journal of Oceanography In Taiwan Strait,2011,30(1):138-141. | |
| Authors: | FAN Run-zhen LIN Shu-qun YANG Su-ping SONG Wen-dong |
| Institution: | 1(1.College of Science of Guangdong Ocean University,Zhanjiang 524025,China;2.Shantou MEBO Pharmaceutical Co.,Ltd,Shantou 515041,China;3.Department of Basic Agronomy,Hebei North University,Zhangjiakou 075000,China) |
| Abstract: | Polarographic characteristics of flavones from the leaves of Kandelia candel were studied and the contents of the flavones were determined.The results showed that a sensitive second derivative polarographic wave was produced at the potential of Ep-1 642 mV(vs.SCE) in a solution of borax with a pH of 9.18,in which rutin was used as a reference.The current peak(Ip) of the polarographic wave showed a good linear proportional relationship to rutin,with the rutin concentration being less than 1.4 mg/dm3.Therefore,a polarographic method for the quantitative determination of flavones in the leaves of Kandelia candel was established.The detection limit of this method was 0.02 mg/dm3.Overall,2.04% of the flavones in the Kandelia candel leaves were determined by this method with recoveries in the range of 96.4%～100.3% and an relative standard deviation of 3.19%.This method was sensitive,accurate and subject to little interference. |
| Keywords: | marine chemistry Kandelia candel flavones second derivative linear-sweep polarography |
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