| 东海原甲藻的分子鉴定 |
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| 引用本文： | 罗立明,胡鸿钧,李夜光,齐雨藻,吕颂辉,耿亚红,邓光.东海原甲藻的分子鉴定[J].海洋学报,2006,28(1):127-131. |
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| 作者姓名： | 罗立明 胡鸿钧 李夜光 齐雨藻 吕颂辉 耿亚红 邓光 |
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| 作者单位： | 1.中国科学院武汉植物园/武汉植物研究所, 湖北武汉 430074 |
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| 基金项目： | 国家重点基础研究发展规划资助项目(2001CB409701) |
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| 摘 要： | 采用nrDNAITS序列及18S序列两种分子标记,对东海原甲藻和美国国家海洋藻种保藏中心(CCMP)的具齿原甲藻进行分子鉴定,结果表明,两者之间序列非常相似,两者的nrDNAITS序列碱基差异值仅为0.002,nrDNA18S序列的碱基差异值为0.000,根据分子数据,东海原甲藻和美国国家海洋藻种保藏中心的具齿原甲藻应为同一个种.从基因库获取原甲藻的另外3个种nrD-NAITS序列和8个种的18S序列,用计算机软件进行分析和构建分子系统树,结果显示18S序列用于原甲藻的分子鉴定过于保守,而nrDNAITS更适合于该种属界定的分子标准.
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| 关 键 词： | 东海原甲藻 具齿原甲藻 nrDNAITS nrDNA18S 分子鉴定 |
| 文章编号： | 0253-4193（2006）01-0127-05 |
| 收稿时间： | 09 5 2004 12:00AM |
| 修稿时间： | 2004-09-052004-11-25 |
Molecular identification on Prorocentrum donghaiense |
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| LUO Li-ming,HU Hong-jun,LI Ye-guang,QI Yu-zao,L&#; Song-hui,GENG Ya-hong and DENG Guang.Molecular identification on Prorocentrum donghaiense[J].Acta Oceanologica Sinica (in Chinese),2006,28(1):127-131. |
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| Authors: | LUO Li-ming HU Hong-jun LI Ye-guang QI Yu-zao L&#; Song-hui GENG Ya-hong and DENG Guang |
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| Institution: | 1.Wuhan Institute of Botany/Wuhan Botanic Garden, Chinese Academy of Sciences, Wuhan 430074, China2.Institute of Hydrobiology, Jinan University, Guangzhou 520632, China |
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| Abstract: | Prorocentrum donghaiense and P.dentatum from CCMP were re-identified by two molecular makers) nrDN AITS and 18S sequences.The sequences of these two species are very similar.The results show that the nrDNA ITS nucleotide divergence between P.donghaiense and P.dentatum CCMP is only 0.002 and 18S nucleotide divergence is 0.000.According to the molecular data,P.donghaiense and P. dentatum CCMP are a similar species.Other three nrDNA ITS sequences and eight 18S sequences of Prorocentrum were obtained from the Genbank.These sequences were analyzed and the phyogenetic trees were constructed by computer programme(Clustal X and M ega).The results show that an nrDNA ITS region is a more suitable molecular maker to identifying interspecies of Prorocentrum and 18S sequence is too conservative. |
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| Keywords: | Prorocentrum donghaiense P dentatum nrDNA ITS nrDNA 18S molecular identification |
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