| Ultrastructure of Single Cells ,Callus—like and Monos—pore—like Cells in Porphyra yezoensis Ueda on Semi—slid Culture Medium |
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| 引用本文： | 梅俊学,沈颂东,姜明,费修绠.Ultrastructure of Single Cells ,Callus—like and Monos—pore—like Cells in Porphyra yezoensis Ueda on Semi—slid Culture Medium[J].中国海洋与湖沼学报,2003,21(2):166-170. |
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| 作者姓名： | 梅俊学 沈颂东 姜明 费修绠 |
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| 作者单位： | [1]MarineBiotechnologyDepartment,ShandongUniversityWeihaiBranch,Weihai264209,China [2]BiologyDepartment,SuzhouUniversityWestCampus,Suzhou215151,China [3]QingdaoOceanUniversity,Qingdao266003,China [4]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 摘 要： | It had been demonstrated that individual or protoplasts isolated from Porphyra thallus by enzyme could develop into normal leafy thalli in the same way as monosproes,and that isolated cells develop in different way in liquid and on semi-solid media,The authors observed the ultrastructure of isolated vegetative cells cultured on semi-solid media and compared them with those of monospores and isolated cells cultured in liquid media,The results showed that subcellular struce-tures were quite different among cells in different conditions.In their development,isolated cells on semi-solid media did not show the characteristic subcellular eature of monospore formation ,such as production of fibrous vesicles,Callus-like cells formed on semi-solid media underwent a distinctive modification in cellular organization.They developed characteristic cell inclusions and a special 2-layer cell covering,Golgi bodies,ER ,starch grains,mitochondria,Vaculoes were not commonly found in them.
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| 关 键 词： | 超微结构 细胞 单孢子 紫菜 原生质体 |
Ultrastructure of single cells,callus-like and monospore-like cells in<Emphasis Type="Italic">Porphyra yezoensis</Emphasis> ueda on semisolid culture medium |
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| Mei?Junxue,Shen?Songdong,Jiang?Ming,Fei?Xiugeng.Ultrastructure of single cells,callus-like and monospore-like cells inPorphyra yezoensis ueda on semisolid culture medium[J].Chinese Journal of Oceanology and Limnology,2003,21(2):166-170. |
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| Authors: | Mei Junxue Shen Songdong Jiang Ming Fei Xiugeng |
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| Institution: | 1.Marine Biotechnology Department,Shandong University Weihai Branch,Weihai,China;2.Biology Department,Suzhou University West Campus,Suzhou,China;3.Qingdao Ocean University,Qingdao,China;4.Institute of Oceanology,Chinese Academy of Sciences,Qingdao,China |
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| Abstract: | It had been demonstrated that individual cells or protoplasts isolated fromPorphyra thallus by enzyme could develop into normal leafy thalli in the same way as monospores, and that isolated cells develop in
different way in liquid and on semi-solid media. The authors observed the ultrastructure of isolated vegetative cells cultured
on semi-solid media and compared them with those of monospores and isolated cells cultured in liquid media. The results showed
that subcellular structures were quite different among cells in different conditions. In their development, isolated cells
on semi-solid media did not show the characteristic subcellular feature of monospore formation, such as production of fibrous
vesicles. Callus-like cells formed on semi-solid media underwent a distinctive modification in cellular organization. They
developed characteristic cell inclusions and a special 2-layer cell covering. Golgi bodies, ER, starch grains, mitochondria.
Vacuoles were not commonly found in them. |
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