| 军曹鱼全人工繁殖群体遗传特征的SSR分析 |
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| 引用本文： | 王中铎,陈铁妹,郭昱嵩,罗杰,刘丽,刘楚吾.军曹鱼全人工繁殖群体遗传特征的SSR分析[J].广东海洋大学学报,2010,30(3):16-21. |
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| 作者姓名： | 王中铎 陈铁妹 郭昱嵩 罗杰 刘丽 刘楚吾 |
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| 作者单位： | 广东海洋大学水产学院,南海水产经济动物增养殖广东普通高校重点实验室,广东,湛江,524025 |
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| 基金项目： | "十一五"国家科技支撑计划项目 |
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| 摘 要： | 利用8个微卫星DNA位点分析南海海域5个军曹鱼全人工繁育群体(HN1、HN2、ZJ、FJ和LS)子代的遗传多样性特征和群体间遗传分化。结果显示,军曹鱼养殖群体与天然群体的遗传结构特征基本一致:1)平均有效等位基因数为3.910±0.440,观测杂合度为0.595±0.049,分子方差分析(AMOVA)表明,个体内分化占主导(46%),军曹鱼养殖群体整体遗传多样性较高;2)群体间基因流明显(N_m=2.5959,F_(st)=0.0878),整体分化程度较低。各养殖群体表现出不同于天然大群体的特征:1)绝大部分位点均明显偏离哈温平衡,杂合子缺失或过剩现象普遍存在;2)聚类和群体分配分析等表明HN2与另四个养殖群体(HN1、ZJ、FJ和LS)分化明显。
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| 关 键 词： | 军曹鱼 养殖群体 微卫星DNA 遗传多样性 |
A Genetic Analysis of Cultured Populations of Cobia (Rachycentron canadum) with Microsatellite Markers |
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| WANG Zhong-duo,CHEN Tie-mei,GUO Yu-song,LUO Jie,LIU Li,LIU Chu-wu.A Genetic Analysis of Cultured Populations of Cobia (Rachycentron canadum) with Microsatellite Markers[J].Journal of Zhanjiang Ocean University,2010,30(3):16-21. |
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| Authors: | WANG Zhong-duo CHEN Tie-mei GUO Yu-song LUO Jie LIU Li LIU Chu-wu |
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| Abstract: | Eight microsatellite loci were employed to elucidate genetic diversity of five cobia(Rachycentron canadum) cultured stocks in the South China Sea(named population HN1,HN2,ZJ,FJ and LS).The result showed as follows:the overall genetic characteristics of the total cultured population were consistent with the wild(the mean allele number was 3.910±0.440,and mean observed heterozygosity was 0.595±0.049,and AMOVA revealed the proportion of genetic variation within individual was 46%),and the genetic diversity of cultured populations was moderate with minor decrease.While the obvious deviation from Hardy-Weinberg equilibrium and heterozygote deficiency or excess were observed in each cultured population because of their small founding broodstocks.Although the overall gene flow was obvious for gene flow value N_m was 2.595 9 and the fixation index F_(st) was 0.087 8,two distinct groups of strains(HN2 and the others) were shown by a population assignment analysis and a phylogenic tree. |
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| Keywords: | cobia(Rachycentron canadum) cultured population microsatellite DNA genetic diversity |
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