| 海岸带沉积物环境中甲烷代谢茵的富集培养及混合茵群分析 |
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| 引用本文： | 于丽波,姜丽晶,汤熙翔.海岸带沉积物环境中甲烷代谢茵的富集培养及混合茵群分析[J].台湾海峡,2011,30(3):386-393. |
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| 作者姓名： | 于丽波 姜丽晶 汤熙翔 |
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| 作者单位： | 国家海洋局第三海洋研究所;国家海洋局海洋生物遗传资源重点实验室,福建厦门361005 |
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| 基金项目： | 福建省科技计划重点资助项目(2009N0040) |
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| 摘 要： | 结合Hungate无氧分离技术，对海岸带沉积物中甲烷代谢菌进行了富集培养，分别为珠江口的甲烷产生菌和九龙江口的甲烷氧化菌及其他甲基氧化菌．其研究结果表明：在珠江口淇澳岛海岸带沉积物中，甲烷八叠球菌属（Methanosarcina）为优势菌株，分布于沉积物的上、中、下3个层位，并发现了部分序列与不可培养的泉古菌门（Crcnarchaeota）的杂色泉古菌（miscellaneouscrenarchaeoticgroup，MCG）的相似度为90％～99％．在九龙江口的海岸带沉积物环境中，噬甲基菌属（Methylophaga）为优势菌群，在富集产物菌群多样性中占60％～99％；还有一些相似度较低（为95％～97％）的菌群，为潜在的新种．
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| 关 键 词： | 海洋微生物学 甲烷产生菌 甲基氧化菌 沉积物 杂色泉古菌（MCG） 富集培养 珠江口 九龙江口 |
Enrichment cultivation of methane metabolic bacteria in the environment of sediments from the coastal zones and analysis of mixed bacteria communities |
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| YU Li-bo,JIANG Li-jing,TANG Xi-xiang.Enrichment cultivation of methane metabolic bacteria in the environment of sediments from the coastal zones and analysis of mixed bacteria communities[J].Journal of Oceanography In Taiwan Strait,2011,30(3):386-393. |
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| Authors: | YU Li-bo JIANG Li-jing TANG Xi-xiang |
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| Institution: | YU Li-bo,JIANG Li-jing,TANG Xi-xiang(Key Laboratory of Marine Biogenetic Resources,Third Institute of Oceanography,SOA,Xiamen 361005,China) |
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| Abstract: | Methane(CH4) is one of the main greenhouse gases responsible for global warming.Most methane in the atmosphere is the result of the interaction between Methanogens and Methanotrophs.Therefore,it is of great significance for both energy and environment to research Methanogens and Methanotrophs.In this study,enrichment and biodiversity analyses were conducted on bacteria related to methane metabolism from the coastal zone sediments.The Methanogens from Qi'ao Island in the Pearl River Estuary,China and the Met... |
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| Keywords: | marine microbiology methanogens methyl-oxidizing bacteria sediment miscellaneous crenarchaeotic group(MCG) enrichment culture Pearl River Estuary Jiulongjiang River Estuary |
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