| 通过焦磷酸测序揭示北极海水的微生物群落结构 |
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| 引用本文： | 李阳,王桢,林学政.通过焦磷酸测序揭示北极海水的微生物群落结构[J].海洋学报(英文版),2016,35(6):78-84. |
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| 作者姓名： | 李阳 王桢 林学政 |
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| 作者单位： | 国家海洋局第一海洋研究所, 山东 青岛 266061;国家海洋局海洋生物活性物质重点实验室, 山东 青岛 266061,国家海洋局第一海洋研究所, 山东 青岛 266061;国家海洋局海洋生物活性物质重点实验室, 山东 青岛 266061,国家海洋局第一海洋研究所, 山东 青岛 266061;国家海洋局海洋生物活性物质重点实验室, 山东 青岛 266061 |
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| 摘 要： | This study aimed to determine the microbial community structure of seawater in(ICE-1) and out(FUBIAO) of the pack ice zone in the Arctic region.Approximate 10 L seawater was filtrated by 0.2 μm Whatman nuclepore filters and the environmental genomic DNA was extracted.We conducted a detailed census of microbial communities by pyrosequencing.Analysis of the microbial community structures indicated that these two samples had high bacterial,archaeal and eukaryotic diversity.Proteobacteria and Bacteroidetes were the two dominant members of the bacterioplankton community in both samples,and their relative abundance were 51.29% and 35.39%,72.95%and 23.21%,respectively.Euryarchaeota was the most abundant archaeal phylum,and the relative abundance was nearly up to 100% in FUBIAO and 60% in ICE-1.As for the eukaryotes,no_rank_Eukaryota,Arthropoda and no_rank_Metazoa were the most abundant groups in Sample FUBIAO,accounting for 85.29% of the total reads.The relative abundance of the most abundant phylum in Sample ICE-1,no_rank_Eukaryota and no_rank_Metazoa,was up to 90.69% of the total reads.Alphaproteobacteria,Flavobacteria and Gammaproteobacteria were the top three abundant classes in the two samples at the bacterial class level.There were also differences in the top ten abundant bacterial,archaeal and eukaryotic OTUs at the level of 97% similarity between the two samples.
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| 关 键 词： | 北极海水 焦磷酸测序 微生物群落 |
| 收稿时间： | 3/9/2015 12:00:00 AM |
| 修稿时间： | 8/7/2015 12:00:00 AM |
Microbial community structure of Arctic seawater as revealed by pyrosequencing |
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| LI Yang,WANG Zhen and LIN Xuezheng.Microbial community structure of Arctic seawater as revealed by pyrosequencing[J].Acta Oceanologica Sinica,2016,35(6):78-84. |
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| Authors: | LI Yang WANG Zhen and LIN Xuezheng |
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| Institution: | The First Institute of Oceanography, State Oceanic Administration, Qingdao 266061, China;Key Laboratory of Marine Bioactive Substances, State Oceanic Administration, Qingdao 266061, China |
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| Abstract: | This study aimed to determine the microbial community structure of seawater in (ICE-1) and out (FUBIAO) of the pack ice zone in the Arctic region. Approximate 10 L seawater was filtrated by 0.2 μm Whatman nuclepore filters and the environmental genomic DNA was extracted. We conducted a detailed census of microbial communities by pyrosequencing. Analysis of the microbial community structures indicated that these two samples had high bacterial, archaeal and eukaryotic diversity. Proteobacteria and Bacteroidetes were the two dominant members of the bacterioplankton community in both samples, and their relative abundance were 51.29% and 35.39%, 72.95% and 23.21%, respectively. Euryarchaeota was the most abundant archaeal phylum, and the relative abundance was nearly up to 100% in FUBIAO and 60% in ICE-1. As for the eukaryotes, no_rank_Eukaryota, Arthropoda and no_rank_Metazoa were the most abundant groups in Sample FUBIAO, accounting for 85.29% of the total reads. The relative abundance of the most abundant phylum in Sample ICE-1, no_rank_Eukaryota and no_rank_Metazoa, was up to 90.69% of the total reads. Alphaproteobacteria, Flavobacteria and Gammaproteobacteria were the top three abundant classes in the two samples at the bacterial class level. There were also differences in the top ten abundant bacterial, archaeal and eukaryotic OTUs at the level of 97% similarity between the two samples. |
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| Keywords: | Arctic seawater pyrosequencing microbial community |
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