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| Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns | |
| 作者姓名： | WU Changwen CHI Changfeng HE Guangyuan L&#; Zhenming XU Meiying |
| 作者单位： | Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China;Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China,Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China,Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China,Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China,Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China |
| 基金项目： | The National Key Technology R & D Program of China under contract No. 2007BAD43B05; the Key Science & Technology Specific Projects of Zhejiang Province under contract No. 2007C12062; the National Natural Science Foundation of China under contract No. 31001109 |
| 摘 要： | Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni. |
| 关 键 词： | 曼氏无针乌贼 微卫星多态性 微卫星位点 鉴定 墨鱼 分离 浓缩 多态信息含量 |
| 收稿时间： | 9/2/2009 12:00:00 AM |
| 修稿时间： | 2010/4/20 0:00:00 |
Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns |
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| WU Changwen,CHI Changfeng,HE Guangyuan,L&#; Zhenming,XU Meiying.Isolation via enrichment and characterization of ten polymorphic microsatellite loci in the cuttlefish, Sepiella maindroni de Rochebruns[J].Acta Oceanologica Sinica,2010,29(6):121-124. | |
| Authors: | WU Changwen CHI Changfeng HE Guangyuan L&#; Zhenming and XU Meiying |
| Institution: | 1. Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China;Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China 2. Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China;Zhejiang Dahaiyang Science and Technology CO. Ltd., Zhoushan 316004, China 3. Key Laboratory of Molecular Biophysics, Ministry of Education China-UK HUST-RRes Genetic Engineering and Genomics Joint Laboratory, Huazhong University of Science and Technology, Wuhan 430074, China 4. Marine Science School, Zhejiang Ocean University, Zhoushan 316004, China |
| Abstract: | Ten novel polymorphic microsatellite loci were developed using an enrichment and colony hybridization protocol and characterized for the cuttlefish, Sepiella maindroni. Polymorphism was explored by using 30 individuals from the coastal waters of Zhanjiang, Guangdong province, China, in December 2007. The number of alleles ranged from 5 to 13. The ranges of observed and expected heterozygosity were from 0.578 9 to 1.000 0 and 0.682 8 to 0.925 7, respectively, and the average polymorphic information content (PIC) was 0.778 5. These microsatellite loci will certainly facilitate the detection of the genetic variation and population structure of S. maindroni. |
| Keywords: | Sepiella maindroni microsatellite DNA cuttlefish genetic variation |
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