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对虾组织蛋白质和同工酶表型及其在病虾中的变化
引用本文:王金星,赵小凡,张红卫,于士广,刘昌彬,张文学,高守华.对虾组织蛋白质和同工酶表型及其在病虾中的变化[J].海洋科学,1995,19(3):46-51.
作者姓名:王金星  赵小凡  张红卫  于士广  刘昌彬  张文学  高守华
作者单位:山东大学生物系!济南250100
摘    要:利用SDS-聚丙烯酰胺凝胶电泳和非变性聚丙烯酰胺凝胶龟泳对中国对虾四和中组织的蛋白质、酯酶、乳酸脱氢酶和超氧化物歧化酶等同工酶进行了分析研究,并将健康虾与流行病病虾进行了比较。结果表明,肝胰脏和肌肉组织的蛋白质表型、肝胰脏酯酶和超氧化物歧化酶的表型在健康虾和病虾之间存在着较明显的差异。说明肝胰脏内蛋白质代谢有明显的紊乱现象。肝胰脏和肌肉的组织蛋白、肝酯酶和超氧化物歧化酶的特异性变化,可作为虾病早期

关 键 词:中国对虾  组织蛋白质  同工酶  虾病诊断  对虾
收稿时间:1994/4/13 0:00:00

PHENOTYPES OF TISSUE PROTEINS AND THREE ISOZYMES OF CHINESE PRAWNS Panaeus chinensis AND THEIR PATHOLOGICAL CHANGES IN DISEASED PRAWNS
Wang Jinxing, Zhao Xiaofan, Zhang Hongwei, Yu Shiguang, Liu Changbin, Zhang Wenxue, and Gao Shouhua.PHENOTYPES OF TISSUE PROTEINS AND THREE ISOZYMES OF CHINESE PRAWNS Panaeus chinensis AND THEIR PATHOLOGICAL CHANGES IN DISEASED PRAWNS[J].Marine Sciences,1995,19(3):46-51.
Authors:Wang Jinxing  Zhao Xiaofan  Zhang Hongwei  Yu Shiguang  Liu Changbin  Zhang Wenxue  and Gao Shouhua
Abstract:The phenotypes of tissue proteins, esterase, lactate dehydrogenase and superoxide dismutase of four tissues from healthy and diseased prawns Panaeus chinensis were analysed by means of SDS-PAGE and native polyacrylamid gel electrophoresis. The results show that the phenotypes of hepatopancreas proteins, Est and SOD of healthy prawns are different from those of diseased ones. It provides the biochemical evidence for the mechanism of the epidemic disease. The pathological changes of tissue proteins and two isozymes in diseased prawns are considered to be a biochemical diagnosis marker in the early stage of the disease.
Keywords:Panaeus chinensis  tissue proteins  isozymes diagnosis of epidemic disease  
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