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Ras gene in marine mussels: a molecular level response to petrochemical exposure |
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| Authors: | Lima I Peck M R Rendón-Von Osten J Soares A M V M Guilhermino L Rotchell J M |
| Institution: | a ICBAS - Instituto de Ciências Biomédicas de Abel Salazar, Departamento de Estudos de Populações, Laboratório de Ecotoxicologia, Universidade do Porto, Largo Prof. Abel Salazar 2, 4099-003 Porto, Portugal b CIMAR LA/CIIMAR - Centro Interdisciplinar de Investigação Marinha e Ambiental, Laboratório de Ecotoxicologia, Universidade do Porto, Rua dos Bragas 289, 4050-123 Porto, Portugal c Department of Biology and Environmental Sciences, University of Sussex, Falmer, Brighton, BN1 9QJ, United Kingdom d CESAM - Centro de Estudos do Ambiente e do Mar and Departamento de Biologia da Universidade de Aveiro, Campus Universitário de Santiago, 3810-193 Aveiro, Portugal e Centro EPOMEX, Universidad Autónoma de Campeche, Apdo. Postal 520, Campeche 24030, Campeche, Mexico |
| Abstract: | Mussels are susceptible to numerous toxicants and are often employed as bioindicators. This study investigated the status of the ras proto-oncogene in Mytilus galloprovincialis following petrochemical exposure. A M. galloprovincialis homologue of the vertebrate ras gene was isolated, showing conserved sequence in regions of functional importance and a high incidence of polymorphic variation. Mutational damage was investigated in mussels chronically exposed to the water-accommodated fraction of #4 fuel-oil (WAF), and in mussels collected along the NW coast of Portugal in sites with different levels of petrochemical contamination. A ras gene point mutation was identified in the codon 35 of one individual exposed to 12.5% WAF. No mutations were detected in mussels from the WAF control or environmental samples. This represents the first report of a ras gene mutation, experimentally-induced by petrochemical exposure, in an invertebrate species. |
| Keywords: | Ras gene Mytilus galloprovincialis Mutation Biomarker Genotoxicity Petroleum hydrocarbon |
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