RFLP analysis of a mitochondrial gene,cytochrome oxidase I (COI) of three species of the genusCalyptogena around Japan |
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| Authors: | Shigeaki Kojima Takanori Kobayashi Jun Hashimoto Suguru Ohta |
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| Institution: | (1) Ocean Research Institute, University of Tokyo, 1-15-1 Minamidai, Nakano, 164 Tokyo, Japan;(2) National Institute of Aquaculture, 422-1 Nakatsuhama, 516-01 Nansei, Mie, Japan;(3) Japan Marine Science and Technology Center, 2-15 Natsushima, 237 Yokosuka, Kanagawa, Japan |
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| Abstract: | Phylogenetic relationship among three species of deep-sea vesicomyid bivalvesCalyptogena, i.e.,C. soyoae, C. solidissima, C. fausta and an undescribed species of the Iheya Ridge, Okinawa Trough was analyzed on the basis of the RFLP analysis of the fragment (about 1 kbp) of cytochrome oxidase I. Both the two populations ofC. soyoae (off Hatsushima and Okinoyama Bank, Sagami Bay) consisted of two haplotypes, which could be discriminated by only one restriction site (1.7% sequence divergence).Calyptogena of the Iheya Ridge could not be distinguished from one of the two haplotypes ofC. soyoae. Nucleotide substitution rates between species were calculated and dendrograms were constructed. |
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