Multi-technique approach to characterise the effects of cryopreservation on larval development of the Pacific oyster (Crassostrea gigas) |
| |
| Authors: | S Suneja AB Rusk JR Morrish HR Tervit LT McGowan |
| |
| Institution: | 1. Faculty of Health and Environmental Sciences, School of Applied Sciences, Institute of Applied Ecology New Zealand, Auckland University of Technology, Auckland, New Zealand;2. Cawthron Institute, Nelson, New Zealand;3. AgResearch, Hamilton, New Zealand |
| |
| Abstract: | Cryopreservation experiments were conducted on D-stage larvae of the Pacific oyster (Crassostrea gigas) to investigate the effects of two cryoprotectant solutions and three cooling rates on larval development from 1 to 22 days post-fertilisation. Cryoprotectant solutions were made up to final concentrations (after 1:1 dilution with larvae) of 10% ethylene glycol, 1% polyvinylpyrrolidone and either 0.2 or 0.4 M trehalose. Three cooling rates (0.5, 1 and 2 °C min?1 between ?10 and ?35 °C post-holding) were tested in an orthogonal design with the two cryoprotectants. Results indicate that control larvae out-performed all cryopreservation treatments for survival, feeding consumption and shell length parameters. However, larvae exposed to 0.4 M trehalose did considerably better than those exposed to 0.2 M trehalose, regardless of cooling rate conditions. Scanning electron and light microscopy observations were used to assess larval morphology and organogenesis, indicating that treatments with surviving larvae were morphologically and developmentally similar to control larvae. |
| |
| Keywords: | Crassostrea gigas cryopreservation cryoprotectants organogenesis oyster larval development shell morphology |
|
|
