Effects of ultraviolet radiation on the abundance,diversity and activity of bacterioneuston and bacterioplankton: insights from microcosm studies |
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Authors: | Ana L Santos Isabel Henriques Newton C M Gomes Adelaide Almeida António Correia Angela Cunha |
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Institution: | 1.CESAM (Center for Environmental and Marine Studies), Department of Biology,University of Aveiro,Aveiro,Portugal |
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Abstract: | The effects of ultraviolet-B (0.4 W m−2) radiation on the abundance, diversity and heterotrophic metabolism of bacterioneuston and bacterioplankton communities from
Ria de Aveiro (Portugal) were assessed and compared to those of freshwater communities from Lake Vela (Portugal) in microcosm
experiments. Exposure to 9 h of artificial ultraviolet radiation (UVR) led to 24–33% reduction in bacterial abundance and
up to a 70% decrease in bacterial diversity. Maximum extracellular enzyme activity and monomer incorporation rates were reduced
by 16–90% and 80–100%, respectively. Recovery of bacterial activity during post-UV dark incubations ranged from 10 to 100%
for extracellular enzyme activity and 40% for monomer incorporation rates. In general, the heterotrophic activity of bacterioneuston
was more inhibited by UVR than that of bacterioplankton. However, DGGE profiles revealed greater UVR-induced reductions in
the diversity of bacterioplankton compared to bacterioneuston. The similarity between bacterioneuston and bacterioplankton
communities in samples collected at early morning was lower than at noon (pre-exposed communities) and increased upon experimental
irradiation, possibly indicating selection for UV-resistant bacteria. The observation that UV exposure resulted in enhanced
reduction of bacterioneuston activity, but a lower reduction in bacterial diversity accompanied by enhanced dark recovery
potential compared to bacterioplankton, indicates re-directioning of bacterioneuston metabolism towards stress defence/recovery
strategies rather than the sustained heterotrophic metabolism. Our results indicate that UVR can significantly decrease the
abundance, diversity and activity of bacteria inhabiting the surface and sub-surface layers of freshwater and estuarine systems
with potentially important impacts on the biogeochemical cycles in these environments. |
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