| 摄食紫菜粉等4种单一饵料的蚤状潘脂肪酸组成的比较 |
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| 引用本文： | 刘飞,徐善良,王丹丽,周伟,程亮.摄食紫菜粉等4种单一饵料的蚤状潘脂肪酸组成的比较[J].台湾海峡,2014(1):90-95. |
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| 作者姓名： | 刘飞 徐善良 王丹丽 周伟 程亮 |
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| 作者单位： | [1]宁波大学海洋学院,浙江宁波315211 [2]宁波大学应用海洋生物技术教育部重点实验室,浙江宁波315211 |
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| 基金项目： | 基金项目：国家自然科学基金资助项目（31172043）;浙江省自然科学基金资助项目（LY12C19003）;宁波市重大攻关项目资助项目（2012C10020） |
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| 摘 要： | 用GC／MS法分析比较了紫菜粉、小球藻、酵母、Banta液（牛粪1．5g＋干稻草2g＋沃土20g＋水1000cm3）4种食物条件下蚤状潘的脂肪酸组成．结果表明：4种食物培养下，蚤状潘的总脂含量依次为39．80、60．10、44．60、52．30mg／g．一共检测出脂肪酸14种，其中8种饱和脂肪酸（SFA），2种单不饱和脂肪酸（MUFA），4种多不饱和脂肪酸（PUFA）．紫菜粉培养组与小球藻组PUFA含量显著高于酵母组和Banta液组（P〈0．05），占总脂肪酸的1／3以上，酵母组MUFA质量分数最高（P〈0．05），达55．73％，Banta液组以SFA质量分数最高（P〈0．05），为52．45％．其中紫菜粉组的PUFA中缺乏C18：3，但C20：5（EPA）和C20：4（AA）质量分数高达22．67％和9．02％是小球藻组的1．6倍和4．5倍（P〈0．05），而且EPA和AA的实际含量也显著高于小球藻组（P〈0．05），达到9．02mg／g和3．59mg／g；比较得出，紫菜粉培养组的20碳以上必需不饱和脂肪酸含量丰富，明显高于其他3组．本实验证实蚤状潘的脂肪酸组成与食物脂肪酸成正相关．分析认为，用紫菜粉培养枝角类用于海水育苗会取得更佳的营养效果．
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| 关 键 词： | 海洋生物学 食物 紫菜粉 蚤状澄 脂肪酸 |
Comparisons of fatty acid fed with porphyra composition in Daphnia pulex powder and 3 others |
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| LIU Fei,XU Shan-liang,WANG Dan-li,ZHOU Wei,CHENG Liang.Comparisons of fatty acid fed with porphyra composition in Daphnia pulex powder and 3 others[J].Journal of Oceanography In Taiwan Strait,2014(1):90-95. |
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| Authors: | LIU Fei XU Shan-liang WANG Dan-li ZHOU Wei CHENG Liang |
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| Institution: | 1 Marine College of Ningbo University, Ningbo 315211, China ;2 . Key Laboratory of Applied Marine Biotechnolog, Ministry of Education, Ningbo University, Ningbo 315211, China) |
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| Abstract: | The compositions of fatty acids of water flea Daphnia pulex cultured in micro-particles seaweed powder, Chlorella sp. ,bread yeast and Banta （1.5 g cow dung; 2 g rice straw; 20 g good soil; 1 000 cm3 water） ,were determined by GC/MC. The total lipid level was 39.80 mg/g,60.10mg/g,44.60 mg/g and 52.30 mg/g,respectively. Fourteen fatty acids were found as 8 saturated fatty acids （SFA） ,2 monounsaturated fatty acids （MUFA） ,and 4 polyunsaturated fatty acids （PUFA）. The PUFA of seaweed powder and chlorella group was significantly higher than that of bread yeast and Banta group （p 〈0.05） ,which accounted for more than 1/3 total fatty acids. It found that PUFA was lack of C 18 ： 3 in the seaweed powder group, but EPA was up to 22.67 % （p 〈 0.05 ） , AA up to 9.02% （p 〈 0.05 ） , which was 1.6 times and 4.5 times of Chlorella group. And the actual EPA and AA contents were significantly higher than chlorella group （p 〈 0.05 ）, reached 9.02mg/g and 3.59mg/g. The Daphnia pulex fed with liquid of Banta had 52.45% （p 〈 0.05 ） of SFA while that fed with bread yeast had 55.73% （p 〈 0.05 ） of MUFA. By comparison, we found that the unsaturated fatty acids up 20 were rich in micro-particles seaweed powder group. This experiment confirmed that the fatty acid composition of Daphnia pulex was positively correlated with its food. It is considered that Daphnia pulex fed with micro-particles seaweed powder has achieved better nutritional effect for breeding in maricultures. |
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| Keywords: | marine biology food seaweed powder Daphnia pulex fatty acids |
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