Isozyme expression of Chinese and Japanese populations of Chlamys farreri and their reciprocal hybrids |
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| Authors: | Li Taiwu Liu Yan Song Linsheng and Sun Xiuqin |
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| Institution: | (1) Faculty of Life Science and Technology, Ningbo University, 315211 Ningbo, China;(2) Ningbo Polytechnic College, 315800 Ningbo, China;(3) Institute of Oceanology, The Chinese Academy of Sciences, 266071 Qingdao, China;(4) First Institute of Oceanography, State Oceanic Administration, 266003 Qingdao, China |
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| Abstract: | Chinese and Japanese population ofChlamys farreri and their reciprocal hybrids were surveyed in isozyme variability at 13 loci by polyacrytamide gel electrophoresis (PAGE).
Isozyme banding patterns indicated these hybrids were diploid. Loci that were observed as being monomorphic in inbred populations
ofC. farreri were also found to be monomorphic in filial progeny; loci that observed to be polymorphic in parental type generations were
also polymorphic in hybrid generations. Differences existed among allelic frequency of the four types of cross. Within the
reciprocal hybrids the expression of malic enzyme (ME) isozyme was sufficient to distinguishing individual hybrids because
of the band, Rf=0.38. However, there were no noticeable variations among all the samples to differentiate one from another.
Inbreeding was likely to be the main problem in aquaculture. The introduction of new broodstock can improve the genetic diversity.
Hybrid vigor has manifested to a certain extent in the present study.
Supported by the National “973” Program (G1999012008) and “863” Program (2002AA626020). |
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| Keywords: | self-bred progeny filial progeny isozyme inbreeding |
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