Metal pollution assessment of the marine environment by determination of metal-binding proteins in Mytilus sp. |
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| Authors: | J Pavi
i
M &#x;kreblin B Raspor M Branica M Tu&#x;ek-Z&#x;nidari
I Kregar P Stegnar |
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| Institution: | J Pavi?i?, M ?kreblin, B Raspor, M Branica, M Tu?ek-Z?nidari?, I Kregar,P Stegnar |
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| Abstract: | Adult mussels (Mytilus galloprovincialis) (shell length 5–7 cm) exposed to elevated concentrations of Cd (0.1–1.3 mg l−1), in a flowing seawater aquarium under laboratory conditions, responded by elevated synthesis of metal-binding proteins in a digestive gland. Response depended on the Cd concentration level as well as on duration of the exposure time. Results obtained show that inducible proteins (isolated by gel-filtration column chromatography and characterized by electrochemical and spectrophotometric methods) should be considered metallothionein-like proteins.The induced metal-binding proteins of mussels in specific organs or tissues are considered to be a potential biological indicator of metal pollution, taking into account specific qualities of the mussel response to different metals. The advantages of the analytical methods applied (gel-chromatography and polarography) and the relevance of the laboratory data when transferred to environmental conditions are discussed. Previously reported results dealing with metal-binding proteins of Mytilus species are also summarized. |
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