| CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES |
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| 作者姓名： | 郭华荣 张士璀 李红岩 童裳亮 相建海 |
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| 作者单位： | [1]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China [2]OceanUniversityofQingdao,Qingdao266003,China |
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| 基金项目： | ThisworkwassupportedbyChinaMinistryofScienceandTechnology (ContributionNo .81 9 0 4 0 6) |
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| 摘 要： | Three continuous marine fish cell lines of FG (i. e., Hounder Gill) from flounder (Paralichthys olivaceus) gill, SPH (i. e. , Sea Perch Heart) from sea perch (Lateolabrax japonicus) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream (Pagrosomus major) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns wasrelated to morphological changes of fish cells in vitro.
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| 关 键 词： | 乳酸盐 脱氢酶 同工酶 生态学 鳍鱼 细胞形态学 比目鱼 |
| 收稿时间： | 10 July 2006 |
Characterization of lactate dehydrogenase isozyme pattern and morphology of three marine fish cell lines |
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| Guo Hua-rong,Zhang Shi-cui,Li Hong-yan,Tong Shang-liang,Xiang Jian-hai.CHARACTERIZATION OF LACTATE DEHYDROGENASE ISOZYME PATTERN AND MORPHOLOGY OF THREE MARINE FISH CELL LINES[J].Chinese Journal of Oceanology and Limnology,2002,20(3):226-231. |
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| Authors: | Guo Hua-rong Zhang Shi-cui Li Hong-yan Tong Shang-liang Xiang Jian-hai |
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| Institution: | (1) Key Lab For Sustainable Utilization of Marine Fisheries Resources, Ministry of Agriculture, Yellow Sea Fisheries Research Institute, Chinese Academy of Fishery Sciences, Qingdao, 266071, China; |
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| Abstract: | Three continuous marine fish cell lines of FG (i.e., Flounder Gill) from flounder ( Paralichthys olivaceus) gill, SPH (i.e., Sea Perch Heart) from sea perch ( Lateolabrax japonicus ) heart and RSBF (i.e., Red Sea Bream Fin) from red sea bream ( Pagrosomus major ) fin, were characterized by lactate dehydrogenase (LDH) isozyme and morphological analysis. The LDH isozyme patterns of these three cell lines and their corresponding tissues of origin were investigated and compared. The results showed: (1) No difference was found in the LDH isozyme patterns of FG and flounder gill tissue. However, the LDH isozyme patterns of SPH and RSBF were significantly different from their corresponding tissues of origin; (2) LDH isozyme patterns of FG, SPH and RSBF were markedly different from each other and could serve as genetic markers for species identification and detection of cross contamination. Morphological change analysis of these three cell lines in comparison to their original tissues indicated that FG cells still appeared epithelioid without morphological transformation. However, morphological changes were found in SPH and RSBF compared to their original tissues. Therefore, the cellular morphology was still plastic in the relatively stable culture conditions, and it was possible that change of LDH patterns was related to morphological changes of fish cells in vitro . |
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| Keywords: | cell line lactate dehydrogenase cellular morphology flounder sea perch red sea bream |
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