| SCREENING OF AGGLUTININS IN MARINE ALGAE FROM FUJIAN COAST OF CHINA |
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| 作者姓名： | 郑怡 卢海声 |
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| 作者单位： | BioengineeringCollege,FujianTeachersUniversity,Fuzhou350007,China |
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| 基金项目： | Thisresearchwasfundedbyaproject(No.2 0 0 76)oftheEducationDepartmentofFujianProvinceandbytheFujianNaturalScienceFund (No .BO1 1 0 0 0 9) |
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| 摘 要： | Thirty-three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined for agglutinins with different animal and human erythrocytes. Protein extracts from 26 species were active against at least one type of the erythrocytes tested. There were 3 species (Grateloupia imbricata, lshigefoliacea and Entermorpha prolifera) whose extracts could agglutinate all the erythrocytes used. The lowest protein concentration required to produce erythrocyte agglutination varied remarkably, from 3.1μg/ml to 500μg/ml . The strongest activity was found in the agglutina-tion of rabbit erythrocytes by Gloiopeltis furcata extract. Inhibition assays performed with nine mono- and bisaccharides indicated that agglutinations of rabbit erythrocytes by extracts of 7 species were inhibited by one or more types of the sugars assayed. The agglutinating activity shown by extracts of most species wasnot affected when the test solution was heated to 90℃, but was lost at 95℃ - 100℃. A few extracts losttheir activity at 60 RS, 65 RS and 75 RS, respectively.
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| 关 键 词： | 凝集素 海藻 福建 沿海地区 红血球 蛋白质 萃取 |
| 收稿时间： | 12 October 2000 |
| 修稿时间： | 20 May 2001 |
Screening of agglutinins in marine algae from Fujian coast of China |
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| Zheng Yi,Lu Hai-sheng.SCREENING OF AGGLUTININS IN MARINE ALGAE FROM FUJIAN COAST OF CHINA[J].Chinese Journal of Oceanology and Limnology,2002,20(3):256-260. |
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| Authors: | Zheng Yi Lu Hai-sheng |
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| Institution: | (1) Bioengineering College, Fujian Teachers University, 350007 Fuzhou, China |
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| Abstract: | Thirty-three species of marine algae belonging to Rhodophyta, Phaeophyta and Chlorophyta from the Fujian coast were examined
for agglutinins with different animal and human erythrocytes. Protein extracts from 26 species were active against at least
one type of the erythrocytes tested. There were 3 species (Grateloupia imbricata, Ishige foliacea andEntermorpha prolifera) whose extracts could agglutimate all the erythrocytes used. The lowest protein concentration required to produce erythrocyte
agglutination varied remarkably, from 3.1 μg/ml to 500 μg/ml. The strongest activity was found in the agglutination of rabbit
erythrocytes byGloiopeltis furcata extract. Inhibition assays performed with nine mono- and bisaccharides indicated that agglutinations of rabbit erythrocytes
by extracts of 7 species were inhibited by one or more types of the sugars assayed. The agglutinating activity shown by extracts
of most species was not affected when the test solution was heated to 90°C, but was lost at 95°C–100°C. A few extracts lost
their activity at 60°C, 65°C and 75°C, respectively.
This research was funded by a project (No. 20076) of the Education Department of Fujian Province and by the Fujian Natural
Science Fund (No. BO110009). |
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| Keywords: | protein extracts agglutinins erythrocyte agglutination marine algae |
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