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Cloning and sequencing of the ferredoxin gene of blue-green algaAnabaena siamensis |
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| Authors: | Shou-dong Li Li-rong Song Yong-ding Liu Jin-dong Zhao |
| Institution: | 1.Institute of Hydrobiology, Chinese Academy of Sciences, 430072, Wuhan, China ;2.School of Life Sciences, Beijing University, 100871, Beijing, China ; |
| Abstract: | The structure gene for ferredoxin,petFI, fromAnabaena siamensis has been amplified by polymerase chain reaction(PCR) and cloned into cloning vector pGEM-3zf(+). The nucleotide sequence ofpetFI has been determined with silver staining sequencing method. There is 96.8% homology between coding region ofpetFI fromA. siamensis and that ofpetFI fromA. sp. 7120. Amino acid sequences of seven strains of blue-green algae are compared. |
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