Assessment of immune parameters of manila clam <Emphasis Type="Italic">Ruditapes philippinarum</Emphasis> in different physiological conditions using flow cytometry |
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| Authors: | Kyung-Il Park Ludovic Donaghy Hyun-Sil Kang Hyun-Ki Hong Young-Ok Kim Kwang-Sik Choi |
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| Institution: | 1.Department of Aquatic Life Medicine, College of Ocean Science and Engineering,Kunsan National University,Gunsan,Korea;2.Department of Marine and Ecological Sciences, College of Arts and Sciences,Florida Gulf Coast University,Fort Myers,USA;3.School of Marine Biomedical Science (POST BK21) and Marine and Environmental Research Institute,Jeju National University,Jeju,Korea;4.Southern Coastal Environment Research Department, South Sea Institute,KORDI,Geoje,Korea |
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| Abstract: | Cellular and humoral immune parameters are often used as biomarkers to trace environmental and physiological stresses in marine
bivalves. In this study, we compared various immune parameters of Manila clams (Ruditapes philippinarum) under normal conditions and under a high level of desiccation, using flow cytometry. The immune parameters analyzed included,
total hemocyte count, hemocyte mortality, hemocyte DNA damage, reactive oxygen species (ROS) production, and phagocytosis
activity. Total hemocyte count, hemocyte DNA damage, and hemocyte mortality were significantly elevated among clams under
high desiccation stress, while phagocytosis activity and spontaneous ROS production were significantly lower compared to those
parameters of the control clams (p<0.05). These data suggest that the immune parameters analyzed in this study well reflect the physiological status of clams. |
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