| CYTOTOXICITY AND GENOTOXICITY OF POLYETHYLENIMINE AND NICKEL CHLORIDE IN RED SEA BREAM（Pagrosomus major）FIN CELL LINE RSBF |
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| 引用本文： | 郭华荣,张士璀.CYTOTOXICITY AND GENOTOXICITY OF POLYETHYLENIMINE AND NICKEL CHLORIDE IN RED SEA BREAM（Pagrosomus major）FIN CELL LINE RSBF[J].中国海洋与湖沼学报,2002,20(4):323-331. |
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| 作者姓名： | 郭华荣 张士璀 |
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| 作者单位： | [1]CollegeofMarineLifeSciences,OceanUniversityofQingdao,Qingdao266003,China [2]InstituteofOceanology,ChineseAcademyofSciences,Qingdao266071,China |
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| 摘 要： | A continuous marine fish cell line RSBF(i.e.Red Sea Bream Fin)was utilized to screen the cytotoxicity and genotoxicity of polyethylenimine(PEI)and nickel cholride(NiCl2)in this study on the deleterious effects of aquatic genotoxins on fish.At the 0.01 to 1μgml concentration tested,PEI had acute toxicity to the treated RSBF cells(IC50=1.12,0.92,0.88 and 0.64μg/ml PEI for time 0 h,24 h,48 h and 72 h after treatment,respectively)and markedly inhibited their proliferation in a dose-dependent manner.At the 0.001 to 5 μmol/L concentration tested,NiCl2 posed no acute toxicity but significantly stimulated their growth(107?214?of control).Random amplified polymorphic DNA(RAPD)technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of the control and treated cells.RAPD analysis indicated that at the concentrations tested,PEI was more genotoxic than NiCl2 to RSBF cells;that there was a slight dose-dependent response in the genotoxic effect of PEI bue not NiCl2;and that RAPD technique might provide a sensitive,non-specif-ic gentoxic endpoint.And the potent cytotoxicity and genotoxicity of PEI on fish cells showed that we should be cautious in utilizing it as gene vector in fish gene transfer and human gene therapy.
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| 关 键 词： | 细胞毒性 遗传毒性 海水鱼类细胞系 RAPD 氯化镍 PEI 聚乙撑亚胺 |
| 收稿时间： | 9 February 2001 |
| 修稿时间： | 23 December 2001 |
Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF |
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| Guo Hua-rong,Zhang Shi-cui.Cytotoxicity and genotoxicity of polyethylenimine and nickel chloride in red sea bream (Pagrosomus major) fin cell line RSBF[J].Chinese Journal of Oceanology and Limnology,2002,20(4):323-331. |
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| Authors: | Guo Hua-rong Zhang Shi-cui |
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| Institution: | (1) Institute of Oceanology, Chinese Academy of Sciences, 266071 Qingdao, China;(2) College of Marine Life Sciences, Ocean University of Qingdao, 266003 Qingdao, China |
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| Abstract: | A continuous marine fish cell line RSBF (i. c. Red Sea Bream Fin) was utilized to screen the cytotoxicity and genotoxicity
of polyethylenimine (PEI) and nickel chloride (NiCl2) in this study on the deleterious effects of aquatic genotoxins on fish. At the 0.01 to 1 μg/ml concentration tested, PEI
had acute toxicity to the treated RSBF cells (IC50=1.12, 0.92, 0.88 and 0.64 μg/ml PEI for time 0 h, 24 h, 48 h and 72 h after treatment, respectively) and markedly inhibited
their proliferation in a dose-dependent manner. At the 0.001 to 5 μmol/L concentration tested, NiCl2 posed no acute toxicity but significantly stimulated their growth (107%–214% of control). Random amplified polymorphic DNA
(RAPD) technique was used to detect the genotoxic effects of PEI and NiCl2 by comparing the RAPD banding patterns of the control and treated cells. RAPD analysis indicated that at the concentrations
tested, PEI was more genotoxic than NiCl2 to RSBF cells; that there was a slight dose-dependent response in the genotoxic effect of PEI but not NiCl2; and that RAPD technique might provide a sensitive, non-specific genotoxic endpoint. And the potent cytotoxicity and genotoxicity
of PEI on fish cells showed that we should be cautious in utilizing it as gene, vector in fish gene transfer and human gene
therapy.
This work was supported by Ministry of Sciences and Technology of China (G1999012006). |
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| Keywords: | fish cell line cytotoxicity genotoxicity polyethylenimine (PEI) nickel chloride (NiCl2) random amplified polymorphic DNA (RAPD) |
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