| 荣成月湖潮间带单细胞趋磁细菌的多样性研究 |
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| 引用本文： | 杜海舰,张蕊,张文燕,徐丛,陈一然,潘红苗,周克,吴龙飞,肖天.荣成月湖潮间带单细胞趋磁细菌的多样性研究[J].海洋学报(英文版),2017,36(2):94-104. |
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| 作者姓名： | 杜海舰 张蕊 张文燕 徐丛 陈一然 潘红苗 周克 吴龙飞 肖天 |
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| 作者单位： | 中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室;中国科学院大学,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室,青岛农业大学资源与环境学院,法国科研中心, 地中海结构生物学微生物学研究所, 细菌化学实验室,中国科学院海洋研究所, 海洋生态与环境科学重点实验室;海洋国家实验室, 海洋生态与环境科学功能实验室 |
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| 基金项目： | The National Natural Science Foundation of China under contract Nos 41606187 and 41276170; the National Natural Science Foundation of China—Shandong Joint Fund for Marine Science Research Centers under contract No. U1406403; the National Natural Science Foundation of China under contract No. 41330962. |
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| 摘 要： | Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake(China), where their abundance reached 103–104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy(HRTEM) analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16 S r RNA gene sequence analysis, we found fourteen operational taxonomic units(OTUs) which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the Gammaproteobacteria.Compared with known axenic and uncultured marine magnetotactic bacteria, the 16 S r RNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to96.2%(97%). The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake.
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| 关 键 词： | 趋磁细菌 磁小体 生物多样性 月湖 潮间带沉积物 |
| 收稿时间： | 2015/11/4 0:00:00 |
| 修稿时间： | 2016/8/23 0:00:00 |
Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China |
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| DU Haijian,ZHANG Rui,ZHANG Wenyan,XU Cong,CHEN Yiran,PAN Hongmiao,ZHOU Ke,WU Long-fei and XIAO Tian.Characterization of uncultivated magnetotactic bacteria from the sediments of Yuehu Lake, China[J].Acta Oceanologica Sinica,2017,36(2):94-104. |
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| Authors: | DU Haijian ZHANG Rui ZHANG Wenyan XU Cong CHEN Yiran PAN Hongmiao ZHOU Ke WU Long-fei and XIAO Tian |
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| Institution: | Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China;University of Chinese Academy of Sciences, Beijing 100049, China,Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China,College of Resources and Environment, Qingdao Agriculture University, Qingdao 266109, China,Aix Marseille Université, CNRS, LCB UMR 7257, Institut de Microbiologie de la Méditerranée, Marseille F-13402, France and Key Laboratory for Marine Ecology and Environmental Sciences, Institute of Oceanology, Chinese Academy of Sciences, Qingdao 266071, China;Laboratory for Marine Ecology and Environmental Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao 266071, China |
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| Abstract: | Marine magnetotactic bacteria were collected from the intertidal sediments of Yuehu Lake (China), where their abundance reached 103-104 ind./cm3. Diverse morphotypes of magnetotactic bacteria were observed, including cocci and oval, vibrio-, spirillum-, rod-, elliptical-, handle- and bar-shaped forms. The magnetococci were the most abundant, and had flagella arranged in parallel within a bundle. The majority of magnetosomes were arranged in one, two or multiple chains, although irregular arrangements were also evident. All the results of high-resolution transmission electron microscopy (HRTEM) analysis show that magnetosome crystals were composed of Fe3O4, and their morphology was specific to particular cell morphotypes. By the 16S rRNA gene sequence analysis, we found fourteen operational taxonomic units (OTUs) which were related to magnetotactic bacteria. Among these, thirteen belonged to the Alphaproteobacteria and one to the Gammaproteobacteria. Compared with known axenic and uncultured marine magnetotactic bacteria, the 16S rRNA gene sequences of most magnetotactic bacteria collected from the Yuehu Lake exhibited sequence identities ranging from 90.1% to 96.2% (<97%). The results indicate that microbial communities containing previously unidentified magnetotactic bacteria occur in the Yuehu Lake. |
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| Keywords: | magnetotactic bacteria magnetosome biodiversity Yuehu Lake intertidal sediments |
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