| Isozymes Analysis of the Golden Cuttlefish Sepia esculenta (Cephalopoda: Sepiidae) |
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| 作者姓名： | ZHENG Xiaodong * ZHAO Jianmin XIAO Shu WANG Rucai WANG Shidang ZHOU Weiwu The Key Laboratory of Mariculture Ministry of Education Ocean University of China Qingdao P..R.China Wendeng Fishery Technical Extension Station Weihai P..R.China |
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| 作者单位： | ZHENG Xiaodong 1)，*，ZHAO Jianmin 1)，XIAO Shu 1)，WANG Rucai 1)，WANG Shidang 2)，ZHOU Weiwu 2) 1) The Key Laboratory of Mariculture，Ministry of Education，Ocean University of China，Qingdao 266003，P..R.China 2) Wendeng Fishery Technical Extension Station，Weihai 264400，P..R.China |
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| 摘 要： | 1 Introduction ThegoldencuttlefishSepiaesculenta,aneriticanddemersalspecies,livesinthedepthof 1 0 - 1 0 0meters,andsometimesinseabottomsand .Itiswidelydistrib utedfromcentralHonshutoVietnamandthePhilip pines .Thegoldencuttlefishisalsoanimportantobjectof…
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Isozymes Analysis of the Golden Cuttlefish Sepia esculenta (Cephalopoda:Sepiidae) |
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| ZHENG Xiaodong ,*,ZHAO Jianmin ,XIAO Shu ,WANG Rucai ,WANG Shidang ,ZHOU Weiwu The Key Laboratory of Mariculture,Ministry of Education,Ocean University of China,Qingdao ,P..R.China Wendeng Fishery Technical Extension Station,Weihai ,P..R.China.Isozymes Analysis of the Golden Cuttlefish Sepia esculenta (Cephalopoda: Sepiidae)[J].Journal of Ocean University of China,2004,3(1). |
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| Authors: | Zheng Xiaodong Zhao Jianmin Xiao Shu WANG Rucai WANG Shidang ZHOU Weiwu |
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| Institution: | 1. The Key Laboratory of Mariculture, Ministry of Education, Ocean University of China, Qingdao 266003, P.R. China
2. Wendeng Fishery Technical Extension Station, Weihai 264400, P. R. China |
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| Abstract: | Thirty nine isozymes in four tissues (mantle muscle, buccal bulb muscle, eye and liver) of Sepia esculenta were screened for enzymatic analysis using starch gel electrophoretic technique. Eighteen enzymes (G3PDH, LDH, MDH, MEP, IDHP, PGDH, GRS, NP, AAT, CK, AK, EST, ALP, ACP, FBP, MPI, GPI and PGM) show strong activities and good convergence in zymogram. They are proved to be suitable genetic markers in Sepia esculenta. Among the tissues used, mantle muscle is the best for electrophoretic analysis of isozymes. Eye and liver are fairly good for some special enzymes, such as LDH, EST, MPI, etc. Twenty six loci are detected. The proportion of polymorphic loci is 0.115 in the Qingdao sample and 0.153 in the Rizhao sample ( P < 0.99 ). The mean values of the observed and expected heterozygosity per locus of Qingdao sample are 0.016 and 0.017 , while those of the Rizhao sample are 0.023 and 0.025 respectively. |
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| Keywords: | cuttlefish Sepia esculenta isozyme electrophoresis genetic diversity |
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