Complete nuclear ribosomal DNA sequence analyses of the black-footed abalone Haliotis iris |
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| Authors: | Zhansheng Guo Leng Han |
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| Institution: | 1. Marine College, Shandong University at Weihai, Weihai, People’s Republic of China;2. Shandong Province Marine Biological Breeding Engineering Technology Research Center, Weihai, China |
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| Abstract: | The black-footed abalone Haliotis iris is an economically important shellfish species in New Zealand. We successfully amplified, sequenced and analysed the complete nuclear ribosomal DNA (nrDNA) of H. iris. The length of the nrDNA was determined to be around 9.6?kb and included, in order, small subunit ribosomal RNA (nrSSU, 1858bp), internal transcribed spacer (ITS, 749?bp), large subunit ribosomal RNA (nrLSU, 3412bp) and an intergenic spacer (IGS, 3560–3662?bp). The nrLSU genes were identical in two individuals, whereas the nrSSU and ITS regions existed at three and four base differences, respectively. The IGS was more variable than the other nrDNA regions. A phylogenetic tree was constructed based on the ITS sequence datasets, which revealed that Haliotidae has two major subclades, mainly distributed in the North Pacific, Europe and Australia. The complete nrDNA sequence will be useful for the classification, phylogeny and breeding of this shellfish. |
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| Keywords: | New Zealand Haliotis iris ribosomal DNA nrSSU nrLSU ITS IGS |
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