Liver cadmium uptake,catalase inhibition and cadmium thionein production in the killifish (Fundulus Heteroclitus) induced by experimental cadmium exposure |
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| Authors: | RJ Pruell FR Engelhardt |
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| Institution: | Biology Department, Southeastern Massachusetts University, North Dartmouth, Mass., USA;Department of Biology, University of Ottawa, Ottawa, Canada K1N 6N5 |
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| Abstract: | Liver catalase activity was used as an index of cadmium poisoning in the killifish, Fundulus heteroclitus, and was found to be inhibited following both in vivo and in vitro exposure to dissolved cadmium at a concentration greater than 1 mg/litre. Pre-exposure at 1 mg Cd/litre prevented catalase inhibition at higher subsequent doses of cadmium. Comparison of the effects of in vivo and in vitro exposure showed in vivo exposure to cadmium to result in greater catalase inhibition.Cadmium and copper binding was determined by gel chromatography and found to occur at three distinct liver cytosol fraction peaks. Both metals showed enhanced cytosol concentrations with cadmium exposure and relative changes in peak levels as related to cadmium exposure regimes. Cadmium thionein (molecular weight of 7–10,000) was found in the killifish liver and was correlated with adaptive responses in liver catalase activity. High molecular weight cadmium binding was found to be a better index of cadmium toxicity to liver catalase than total liver cadmium concentration. |
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