| 红笛鲷脾脏、头肾和胸腺中IgM~+细胞与ANAE~+ T细胞的分布 |
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| 引用本文： | 李珠,吴灶和,简纪常,鲁义善.红笛鲷脾脏、头肾和胸腺中IgM~+细胞与ANAE~+ T细胞的分布[J].广东海洋大学学报,2007,27(3):55-59. |
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| 作者姓名： | 李珠 吴灶和 简纪常 鲁义善 |
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| 作者单位： | 广东海洋大学,水产学院,广东省水产经济动物病原生物学及流行病学重点实验室,广东,湛江,524025 |
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| 摘 要： | 采用ANAE组化方法(α-醋酸萘酯酶染色法)和ABC免疫组化方法(亲合素-生物素-过氧化物酶复合物法)分析了1龄红笛鲷成鱼的胸腺、头肾和脾脏中IgM+细胞(具有免疫球蛋白分子IgM的细胞)、ANAE+ T细胞的分布。结果表明,三个淋巴器官皆有IgM+细胞、ANAE+ T细胞;胸腺皮质区IgM+细胞数量最多,其次是头肾、胸腺髓质区、脾脏;ANAE+ T细胞以脾脏中数量居多,其次是头肾、胸腺髓质区、胸腺皮质区;ANAE+ T细胞在三个器官中多呈均匀分布,而IgM+细胞在血管、胸腺小体、黑色素巨嗜细胞中心等的外周呈密集分布。实验结果进一步证明了胸腺中的T细胞多为不成熟的T细胞,此外,还含有大量的IgM+细胞;而脾脏、头肾内的ANAE+ T细胞多于胸腺,推测胸腺成熟的T淋巴细胞迁移到头肾和脾脏。
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| 关 键 词： | 红笛鲷 胸腺 头肾 脾脏 IgM+细胞 ANAE+ T细胞 |
| 文章编号： | 1673-9159（2007）03-0055-05 |
| 修稿时间： | 2007-03-20 |
Distribution of IgM+ and ANAE+ T Cells in Spleen, Head Kidney and Thymus of Hunphead Snapper (Lutjanus sanguineus Cuvier) |
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| LI Zhu,WU Zao-he,JIAN Ji-chang,LU Yi-shan.Distribution of IgM+ and ANAE+ T Cells in Spleen, Head Kidney and Thymus of Hunphead Snapper (Lutjanus sanguineus Cuvier)[J].Journal of Zhanjiang Ocean University,2007,27(3):55-59. |
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| Authors: | LI Zhu WU Zao-he JIAN Ji-chang LU Yi-shan |
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| Institution: | Guangdong provincial key laboratory of Pathogen biology and Epidemiology of Aquatic Economic Animal, Fisheries College of Guangdong Ocean University, Zhanj iang , 524025, china |
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| Abstract: | The distributions of IgM and ANAE T cells in spleen,head kidney and thymus of Hunphead snapper(Lutjanus sanguineus Cuvier) were analyzed by using ANAE histochemistry straining,ABC Immunohistochemistry straining.The results indicated that IgM cells and ANAE T cells appeared in three lympoid organs.The number of the IgM cells was ranked in turn in cortex of thymus,head kidney,medulla of thymus and spleen,while the number of ANAE T cells was did sequentially in spleen,head kidney,medulla of thymus and cortex of thymus.ANAE T cells distributed more evenly in these organs,while IgM cells did more densely around vessels,thymic corpuscles and melanamacrophage centres.These data further demonstrated that most of T cell in the thymus were immature,additionally,lots of IgM cells appeared in thymus.The numbers of ANAE T cells in the spleen and head kidney were more than those of thymus,therefore,it is suggested that the matured T cells are emigrated thymus to spleen and head kidney. |
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| Keywords: | Lutjanus sanguineu thymus head kidney spleen IgM cells ANAE T cells |
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