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| 两组不同饲料对中华绒螯蟹(Eriocheir sinensis)卵巢发育及卵黄发生的激素调控 | |
| 引用本文： | 陆剑锋,常国亮,吴旭干,杨筱珍,赵维信,成永旭.两组不同饲料对中华绒螯蟹(Eriocheir sinensis)卵巢发育及卵黄发生的激素调控[J].海洋与湖沼,2010,41(4):505-512. |
| 作者姓名： | 陆剑锋 常国亮 吴旭干 杨筱珍 赵维信 成永旭 |
| 作者单位： | 1. 合肥工业大学生物与食品工程学院,合肥,230009;上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306 2. 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306;淮阴师范学院生命科学学院,淮阴,223300 3. 上海海洋大学省部共建水产种质资源发掘与利用教育部重点实验室,上海,201306 |
| 基金项目： | 国家自然科学基金，上海市农委攻关项目，上海高校创新团队(第二期)资助;2010-2012，国家农业科技成果转化基金 |
| 摘 要： | 甲壳动物的卵巢发育及卵黄发生受到体内各种内分泌激素的调控。本文研究了两组不同饲料—脂类营养平衡组(A组,含有磷脂和HUFA)和不平衡组(B组,缺乏磷脂和HUFA)对中华绒螯蟹卵巢发育及卵黄发生的激素调控,运用放射免疫法和放射化学法分别测定了处于不同卵黄发生期的中华绒螯蟹的肝胰腺、卵巢、血淋巴中孕酮(PG)、雌二醇(E2)的含量及中华绒螯蟹大颚器(MO)合成与分泌甲基法尼酯(MF)的速率。结果表明:1)中华绒螯蟹体内三种组织(肝胰腺、卵巢和血淋巴)中均存在PG和E2,PG可能为E2的前体;2)早熟蟹的MO合成MF速率远远大于正常发育的幼蟹,MF的大量合成与分泌是促使早熟蟹产生的主要内分泌因素;3)B组饲料(含猪油)喂养的河蟹其MO合成MF的速率均高于A组饲料(含鱼油),且B组饲料的早熟蟹转化PG速度比B组饲料的早熟蟹快;4)PG和E2的脂类结合部位能结合外源性卵黄蛋白原(Vg),通过血淋巴的运输被卵泡细胞胞吞,最终与卵母细胞内的内源性Vg结合,共同形成卵黄体;5)多不饱和脂肪酸(如EPA和DHA)可能会抑制类固醇激素的产生。研究表明,MF是中华绒螯蟹的促性腺激素,具有促使PG合成和E2转化的作用,它们共同诱导河蟹卵巢发育及卵黄发生。 |
| 关 键 词： | 中华绒螯蟹 卵巢发育 卵黄发生 早熟 孕酮 雌二醇 甲基法尼酯 |
| 收稿时间： | 2009/12/10 0:00:00 |
| 修稿时间： | 2010/3/12 0:00:00 |
HORMONAL REGULATIONS OF OVARIAN DEVELOPMENT AND VITELLOGENESIS IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS FED ON TWO DIFFERENT DIETS |
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| LU Jian-Feng,CHANG Guo-Liang,WU Xu-Gan,YANG Xiao-Zhen,ZHAO Wei-Xin and CHENG Yong-Xu.HORMONAL REGULATIONS OF OVARIAN DEVELOPMENT AND VITELLOGENESIS IN CHINESE MITTEN CRAB ERIOCHEIR SINENSIS FED ON TWO DIFFERENT DIETS[J].Oceanologia Et Limnologia Sinica,2010,41(4):505-512. | |
| Authors: | LU Jian-Feng CHANG Guo-Liang WU Xu-Gan YANG Xiao-Zhen ZHAO Wei-Xin and CHENG Yong-Xu |
| Institution: | School of Biotechnology and Food Engineering, Hefei University of Technology;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,School of Life Sciences, Huaiyin Normal University;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources,;Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, |
| Abstract: | crustaceans, ovarian development and vitellogenesis are known to be regulated by circulating hormones. This study examined the hormonal regulations of ovarian development and vitellogenesis in Chinese mitten crab Eriocheir sinensis fed on two different diets (group A, lipid nutrition balance; group B, lipid nutrition unbalance). The concentrations of progesterone (PG) and estradiol (E2) in the hepatopancreas, ovary, and hemolymph of E. sinensis were measured at different vitellogenic stages by radioimmunoassay. Methyl farnesoate (MF), biosynthesized and secreted by mandibular organ (MO), were analyzed by radiochemical method. The main results were as follows: 1) Occurrence of PG and E2 in the hepatopancreas, ovary and hemolymph, and PG might be the precursor of E2; 2) MF biosynthesis rates of precocious crabs were markedly higher than those of normal juvenile crabs, and large amounts of MF biosynthesized and secreted by MO were the endocrine basis for precocity; 3) MF biosynthesis rates of group B (diets containing pork lard ) were higher than those of group A (diets containing fish oil and soy lecithin), and conversion rates of PG to E2 of group B were also higher than those of group B; 4) PG and E2 possibly bound to Exogenous vitellogenin (Vg) at their lipid binding sites, and were then transported via haemolymph to follicle cells via receptor-mediated endocytosis, and finally integrated with endogenous Vg of oocytes, resulting in their incorporation into the yolk; 5) PUFA such as EPA and DHA might inhibit the production of steroid hormone. Results of this study indicated that MF might act as a gonadotropin which stimulated PG synthesis and E2 conversion, and these three hormones together induced ovarian development and vitellogenesis. |
| Keywords: | Eriocheir sinensis Ovarian development Vitellogenesis Precocity Progesterone Estradiol Methyl farnesoate |
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