Promotive effect of se on the growth and antioxidation of a blue-green algaSpirulina maxima |
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| Authors: | Zhou Zhi-gang Liu Zhi-li |
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| Institution: | (1) Department of Biological Science and Technology, Nanjing University, 210093 Nanjing;(2) Present address: The College of Fisheries, Shanghai Fisheries University, 200090 Shanghai |
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| Abstract: | Cultures of a blue-green algaSpirulina maxima (Setch. et Gard.) Geitler with various concentrations of Se in Zarrouk's medium showed that not higher than 40 mg/L Se could
promote its growth. The present experiments showed thatS. maxima grown under normal conditions, has an oxidant stress defence system for hydrogen peroxide (H2O2) removal, which is the Halliwell-Asada pathway. When 4 to 20 mg/L Se was added to the algal medium, this pathway was replaced
by a so-called Sestressed pathway containing GSH peroxidase (GSH-POD). As a result of the occurrence of both higher activity
of GSH-POD and lower levels of hydroxyl radical (OH·), the Se-stressed pathway scavenged H2O2 so effectively that the growth ofS. maxima was promoted by 4 to 20 mg/L Se. While GSH-POD activity of the alga disappeared at 40 mg/L Se, the recovery of ascorbate
peroxidase was observed. The lower levels of ascorbic acid and GSH made the Halliwell-Asada pathway for scavenging H2O2 less effective, while the highest activity of catalase might be responsible in part for the H2O2 removal, causing the level of OH· inS. maxima grown at 40 mg/L Se to be much higher than the OH· level in this alga grown at 4 to 20 mg/L Se, but lower than that in the
control. The OH· level changes caused the growth ofS. maxima cultured at 40 mg/L Se to increase slightly to close to that of the control. |
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| Keywords: | ascorbate peroxidase glutathione peroxidase (GSH-POD) growth hydrogen peroxide (H2O2) hydroxyl radical (OH·) Se Spirulina maxima |
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