| 1854m～2608m气源岩中产甲烷菌的富集培养和发酵产气实验研究 |
| |
| 引用本文： | 丁安娜,连莉文,张辉,张中宁,惠荣耀.1854m～2608m气源岩中产甲烷菌的富集培养和发酵产气实验研究[J].沉积学报,1995,13(3):117-125. |
| |
| 作者姓名： | 丁安娜 连莉文 张辉 张中宁 惠荣耀 |
| |
| 作者单位： | 1 中国科学院兰州地质研究所 兰州 730000; |
| |
| 摘 要： | 供试样品１０个，为准噶尔盆地马庄气藏侏罗系气源岩，埋深１８５４ｍ～２６０８ｍ，形成于沼泽－滨浅湖相沉积环境，为ⅡＢ～Ⅲ型有机质，Ｒ０值＜０.７４％，处于低演化阶段。本文就烃源岩中厌氧发酵细菌的数量及产甲烷菌的类型、分布状况进行了研究，并直接利用地质样品进行了生化模拟产气实验。样品中发酵性细菌数量分布在５×１０２个/ｇ～２.３×１０４个/ｇ之间。在３５℃～６５℃状态下，从样品中普遍富集培养出产甲烷球菌（Ｍｅｔｈａｎｏｃｏｃｕｓ）；在５５℃条件下，从７号样品中富集培养出产甲烷杆菌（Ｍｅｔｈａｎｏｂａｃｔｅｒｉｕｍ）。模拟产气结果表明:①３５℃时的甲烷累计产气量最高，为２９.４８ｍｌ～３９.４１ｍｌ，占总产出气体的８０％以上。（２）产出气体组份一致，以ＣＨ４和ＣＯ２为主，含极微量的乙烷和丙烷。（３）在３５℃时，产出甲烷气体的δ１３Ｃ值分布在－４０.１２‰--４３.９８‰之间，５５℃时为－５６.８２‰--６０.１０‰，６５℃时为－５８.０６‰--６５.１１‰，３５℃～５５℃时产出气体的甲烷碳同位素比值中值与实际气藏天然气的甲烷δ１３Ｃ值相近。该项研究为探讨产甲烷菌等厌氧微生物在２０００多米沉积岩中仍有活动提供了直接?
|
| 关 键 词： | 烃源岩 产甲烷菌 发酵模拟实验 准噶尔盆地 |
| 收稿时间： | 1994-11-10 |
Studies on the Enriched Cultivation of Methanogen and the Fermentation Experiment by Using 1854 to 2608m Gas Source Rocks |
| |
| Institution: | 1 Lanzhou Institute of Geology, Chinese Academy of Sciences, Lanzhou 730000;2 Chengdu Biogas Research Institute of the Ministry of Agriculture, Chengdu 610041 |
| |
| Abstract: | A10 samples were collected from the Jurassic system of the Mazhuang gas reservoir in the Junggar Basin. Formed in a swamp- shallow shore depositional environment, they consist of rich TypeⅡ B and Ⅲ organic matter with R0 values less than 0.74% at the low evolutionary stage. This paper mainly discusses questions on the amount of anerobic fermenting bacteria, the type and distribution of methanogen, and the methane yield of per gram organic carbon in the process of the simulahng fermentation experiment and so on. The numbers of permenting bacteria are from 5×102/g rock to 2.3×104/g rock. At 35℃-65℃,Methanococcus is found in all samples, at 55℃, Methanobocterium is found in No. 7 sample. Simulating experiment results show: (1 )methane accumulation yields are the most at 35℃, from 29.48ml to 39.41 ml, over 80% in the total gas-producing yield. (2)in the gas product, methane is the major, secondly carbon dioxide, and only a trace of ethane and profane is present. (3)from the methane-producing yield of per gram organic carbon, it can be seen that most of samples produce 50ml/g org. to 200ml/g org. gas, and the highest reaches to 568ml/g org. gas. (4)the methaneproducing ability is different at different temperature, 35 ℃ 65 ℃ 55 ℃. (5 )under the condition of the same sample and temprature, with the increase of the methane yield the carbon dioxide yield is also increasing. (6)the δ13C values of methane are - 40.12‰, to - 43.98‰, at 35℃, - 56.82‰ to - 60.10‰, at 55℃, -58.06‰ to - 65.11‰, at 65℃, and the median value of the carbon isotopic ratio of methane at 35℃ to 55 ℃ is nearly similar to the δ13C value of methane from the Mazhuang natural gas reservoir.This poper provides some evidence for that methanogen and other anaerobic microbes still have activity beneath over 2000m sediments and also it gives many significant scientific information to the study on geomicrobiology and some of of natural gas-forming mechnism. |
| |
| Keywords: | |
| 本文献已被 CNKI 等数据库收录！ |
| 点击此处可从《沉积学报》浏览原始摘要信息 |
| 点击此处可从《沉积学报》下载免费的PDF全文 |
|
