| BIOCHEMICAL GENETIC STUDIES ON CUTYLEFISH SEPIELLA MAINDRONI （CEPHALOPODA： SEPIIDAE）——ACTIVE LOCI SCREENING OF ISOZYME |
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| 引用本文： | 郑小东 YutakaNatsukari 王如才 王昭萍 李云.BIOCHEMICAL GENETIC STUDIES ON CUTYLEFISH SEPIELLA MAINDRONI （CEPHALOPODA： SEPIIDAE）——ACTIVE LOCI SCREENING OF ISOZYME[J].中国海洋与湖沼学报,2001,19(4):345-349. |
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| 作者姓名： | 郑小东 YutakaNatsukari 王如才 王昭萍 李云 |
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| 作者单位： | [1]CollegeofFisheries,QingdaoOceanUniversity,Qingdao266003,China） [2]FacultyofFisheries,NagasakiUniversity,Nagasaki,852Japan |
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| 摘 要： | Screening of 46 putative enzyme-coding loci and 4 different kinds of tissues of Sepiella maindroni de Rochebrone, 1884 for enzymatic activities using starch gel electrophoretic technique proved that the 21 enzymes such as AAT, AK, ALP, AP, CK, DIA, ES, FBP, G3PDH, GPI, GRS,IDH, LDH, MDH, MEP, MPI, NP, PGDH, PGM, SOD and XO* , were active to Sepiella maindroni after being stained. The tissue exhibiting stable and clear bands was also determined. Among tissues tested, mantle muscle tissue was the best for electrophoretic survey of isozymes. Buccal bulb muscle, eye and liver were fairly good for some special enzymes, such as DIA, ES, MPI, NT, etc.
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| 关 键 词： | 生物化学 遗传 酶 电泳 褪色 |
| 收稿时间： | 17 August 2000 |
| 修稿时间： | 13 March 2001 |
Biochemical genetic studies on cuttlefishSepiella maindroni (Cephalopoda: Sepiidae)—active loci screening of isozyme |
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| Zheng Xiao-dong,Yutaka Natsukari,Wang Ru-cai,Wang Zhao-ping,Li Yun.Biochemical genetic studies on cuttlefishSepiella maindroni (Cephalopoda: Sepiidae)—active loci screening of isozyme[J].Chinese Journal of Oceanology and Limnology,2001,19(4):345-349. |
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| Authors: | Zheng Xiao-dong Yutaka Natsukari Wang Ru-cai Wang Zhao-ping Li Yun |
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| Institution: | (1) College of Fisheries, Qingdao Ocean University, 266003 Qingdao, China;(2) Faculty of Fisheries, Nagasaki University, 852 Nagasaki, Japan |
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| Abstract: | Screening of 46 putative enzyme-coding loci and 4 different kinds of tissues ofSepiella maindroni de Rochebrone, 1884 for enzymatic activities using starch gel electrophoretic technique proved that the 21 enzymes such as
AAT, AK, ALP, AP, CK, DIA, ES, FBP, G3PDH, GPI, GRS, IDH, LDH, MDH, MEP, MPI, NP, PGDH, PGM, SOD and XO*, were active toSepiella maindroni after being stained. The tissue exhibiting stable and clear bands was also determined. Among tissues tested, mantle muscle
tissue was the best for electrophoretic survey of isozymes. Buccal bulb muscle, eye and liver were fairly good for some special
enzymes, such as DIA, ES, MPI, NP, etc.
This work was funded by the National High-tech Development Plan of China (Grant No. 863-819-01-01). |
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| Keywords: | cuttlefish Sepiella maindroni Cephalopoda isozyme |
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