| 转兔防御素基因小球藻培养的营养模式研究 |
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| 引用本文： | 韩兴梅,李元广,魏晓东,李兴武,孙勇如,王义琴.转兔防御素基因小球藻培养的营养模式研究[J].海洋科学,2006,30(7):48-52. |
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| 作者姓名： | 韩兴梅 李元广 魏晓东 李兴武 孙勇如 王义琴 |
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| 作者单位： | 1. 华东理工大学,海洋生化工程研究所生物反应器工程国家重点实验室,上海,200237
2. 中国科学院,遗传与发育生物学研究所,北京100101 |
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| 基金项目： | 国家863计划海洋生物技术主题资助项目(2002AA629110) |
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| 摘 要： | 在250mL摇瓶中研究了光自养、异养、混合营养3种营养模式对转兔防御素基因(NP-1)小球藻(Chlorella)的生长、NP-1表达量、总蛋白质含量、pH、葡萄糖消耗以及光衰减等几个方面的影响。结果表明,异养培养为转NP-1基因小球藻的最适营养模式。在此基础上,采用5,15,30L生物反应器补料分批异养培养转NP-1基因小球藻,细胞质量浓度分别达到3.67,4.87,6.39g/L。
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| 关 键 词： | 转基因小球藻 兔防御素 营养模式 表达 |
| 文章编号： | 1000-3039（2006）07-0048-05 |
| 收稿时间： | 2004-08-26 |
| 修稿时间： | 2005-08-20 |
Effect of culture modes on the growth of transgenic Chlorella and rabbit defensin expression |
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| HAN Xing-mei,LI Yuan-guang,WEI Xiao-dong,LI Xing-wu,SUN Yong-ru,WANG Yi-qin.Effect of culture modes on the growth of transgenic Chlorella and rabbit defensin expression[J].Marine Sciences,2006,30(7):48-52. |
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| Authors: | HAN Xing-mei LI Yuan-guang WEI Xiao-dong LI Xing-wu SUN Yong-ru WANG Yi-qin |
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| Institution: | 1 .Institute of Marine Bioprocess Engineering, State Key Laboratory of Bioreactor Engineering, East China University of Science and Technology, Shanghai 200237,China; 2.Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101,China |
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| Abstract: | The influences of culture modes including autotrophy, heterotrophy, mixotrophy on process characteristics of transgenic Chlorella with rabbit defensin (NP-1) gene in the 250mL flasks were investigated including cell growth, NP-1expression, protein contents, pH, glucose consumption and light attenuation. The results showed that heterotrophy was the optimal culture mode for the transgenic Chlorella with NP-1 gene. Based on these results, transgenic Chlorella was cultured in bioreactors of 5, 15, 30L and the corresponding cell densities were 3.67, 4.87, 6.39g/L, respectively. |
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| Keywords: | Transgenic Chlorella rabbit defensin culture modes expression |
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