Effect of lead on cytoskeletal protein stability in crucian carp <Emphasis Type="Italic">Carassius auratus</Emphasis> |
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| Authors: | Jia Cheng Dongyi Zhang Wuying Chu Fang Liu Zhen Liu Ruixue Zhou Tao Meng Jianshe Zhang |
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| Institution: | (1) Department of Bioengineering and Environmental Science, Changsha University, Changsha, 410003, China;(2) Core Facilities of Biotechnology, Central South University of Forestry and Technology, Changsha, 410004, China |
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| Abstract: | Inorganic lead (Pb) is one of the most common environmental pollutants. Much evidence indicates that Pb exposure could directly
affect fish growth and development. In this study, we investigated the cytotoxic effects of Pb on cytoskeletal protein stability
at both protein and mRNA level in crucian carp Carassius auratus. Pb(NO3)2 treatment in concentration of 100 μmol/L resulted in decreased expression of both α- and β-tubulin but γ-tubulin as assayed
with SDS-PAGE, Western Blot, and ELISA. In vivo and in vitro analyses on protein expression of tubulins are consistent. The effect of Pb on mRNA expression varied among different tissues.
Our results suggest that cytotoxicity of Pb at protein translation level is stronger than at mRNA expression level.
Supported by National Natural Science Foundation of China (No. 30571414, No 30640015) and Changsha Municipal Project of Science
and Technology (No. K051127-72). |
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| Keywords: | Keyword" target="_blank">Keyword lead crucian carp Carassius auratus tubulin mRNA microtubule |
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