| 马氏珠母贝外套膜培养细胞的电镜观察 |
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| 引用本文： | 王爱民,阎冰,苏琼,叶力.马氏珠母贝外套膜培养细胞的电镜观察[J].海洋科学,2002,26(10):4-9. |
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| 作者姓名： | 王爱民 阎冰 苏琼 叶力 |
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| 作者单位： | 1. 广西海洋研究所,北海,536000;海南大学农学院水产系,海口,570228
2. 广西海洋研究所,北海,536000 |
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| 基金项目： | 国家自然科学基金39560066号，广西自然科学基金匹配资助项目9624010号。 |
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| 摘 要： | 运用扫描电镜和透射电镜对马氏珠母贝（Pinctada martensii D0外套膜组织培养过程中各种细胞的生长变化和活动状况的研究，重点是对上皮细胞分泌活动的观察。最初从外套膜组织迁出的上皮细胞为圆球形，细胞表面近似光滑，但有走向规则细而浅的花纹；培养到第4天，上皮细胞形态上发生了较大的变化，分成A型和B型两种细胞，A型上皮细胞内颗粒物少，是处于合成分泌物的早期阶段或尚未开始合成分泌物，B型上皮细胞处于旺盛的分泌物合成阶段，细胞内粗面内质网和线粒体丰富。培养到15－16天的B型上皮细胞体积明显变大，细胞合成并聚集了大量单层膜包裹的颗粒物，培养到第31天的B型上皮细胞分泌活动减弱，上皮细胞逐渐失去分泌功能，进入衰老，死亡，胞体明显萎缩变小，整个培养过程持续了65d。
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| 关 键 词： | 马氏珠母贝 外套膜 组织培养 透射电镜 扫描电镜 |
| 收稿时间： | 2002/3/11 0:00:00 |
| 修稿时间： | 2002年3月11日 |
ELECTRON MICROSCOPIC OBSERVATIONS ON THE CELLS FROM CULTURED MANTLE OF THE PEARL OYSTER, Pinctada martensii D |
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| WANG Ai-min, YAN Bing SU Qiong YE Li.ELECTRON MICROSCOPIC OBSERVATIONS ON THE CELLS FROM CULTURED MANTLE OF THE PEARL OYSTER, Pinctada martensii D[J].Marine Sciences,2002,26(10):4-9. |
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| Authors: | WANG Ai-min YAN Bing SU Qiong YE Li |
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| Institution: | WANG Ai-min1,2 YAN Bing1 SU Qiong1 YE Li1 |
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| Abstract: | The culture of mantle of the pearl oyster, Pinctada martensii D, has been carried out.Scanning electron microscope(SEM)and transmission electron microscope(TEM)were used to observe the changes of the cells from cultured mantle during the culture. The morphological changes and the secreting activities in the epithelial cells were mainly focused on. The epithelial cells just migrated from the mantle were round and had fine lines on the surface .After 4 day's culture, the epithelial cells could be distinguished morphologically into two types, epithelial cell A ( ECA) and epithelial cell B (ECB). ECA was a primary synthesizing cell or did not start synthesizing. ECB showed active synthesizing and contained a lot of mitochodria and rough endoplasmic reticulum (RER). 15-16 days after initiation of the culture, ECB contained a large number of membrane packed granules ,and secreted the granules by the secretion of break-like. After 31 day's culture, the activities of secretion in ECB became weak and the way of secretion turned to be the granular secretion. After 41 day's culture, the epithelial cells stopped the secretion and got into weak and dying. The whole culture was carried on for 65 days, but no division appeared in the epithelial cells. This paper also discussed why the epithelial cells did. not divide in vitro and how to improve the mediun and the culture technique . |
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| Keywords: | Pinctada martensii D Mantle Tissue culture Scanning electron microscope(SEM) Transmission electron microscope (TEM) |
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