| A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01 |
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| 作者姓名： | 王大志 张树刚 洪华生 |
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| 作者单位： | State Key Laboratory of Marine Environmental Science/Environmental Science Research Center Xiamen University，State Key Laboratory of Marine Environmental Science/Environmental Science Research Center Xiamen University，State Key Laboratory of Marine Environmental Science/Environmental Science Research Center Xiamen University，Xiamen 361005 China，Xiamen 361005 China，Xiamen 361005 China |
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| 基金项目： | 国家自然科学基金;科技部资助项目 |
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| 摘 要： | Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species, Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3’-phosphoadenosine 5’-phosphosulfate (PAPS) to N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2, but is inactive toward STX to produce GTX5. The crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2 , Mg2 , Mn2 and Ca2 individually, but is inhibited by Cu2 . Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.
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| 关 键 词： | 毒性腰鞭毛虫 粗酶提取物 磺基转移酶 麻痹性贝毒 |
| 收稿时间： | 2005-05-25 |
| 修稿时间： | 2006-11-24 |
A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate <Emphasis Type="Italic">Alexandrium tamarense</Emphasis> CI01 |
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| Wang?Dazhi,Zhang?Shugang,Hong?Huasheng.A sulfotransferase specific to N-21 of gonyautoxin 2/3 from crude enzyme extraction of toxic dinoflagellate Alexandrium tamarense CI01[J].Chinese Journal of Oceanology and Limnology,2007,25(2):227-234. |
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| Authors: | Wang Dazhi Zhang Shugang Hong Huasheng |
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| Institution: | (1) State Key Laboratory of Marine Environmental Science/Environmental Science Research Center, Xiamen University, Xiamen, 361005, China |
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| Abstract: | Sulfotransferase (ST) is the first enzyme discovered in association with paralytic shellfish poisoning (PSP) toxin biosynthesis
in toxic dinoflagellates. This study investigates the ST activity in crude enzyme extraction of a toxic dinoflagellate species,
Alexandrium tamarense CI01. The results show that crude enzyme can transfer a sulfate group from 3′-phosphoadenosine 5′-phosphosulfate (PAPS) to
N-21 in the carbamoyl group of gonyautoxin 2/3 (GTX2/3) to produce C1/C2, but is inactive toward STX to produce GTX5. The
crude enzyme is optimally active at pH 6.0 and 15°C. The activity is enhanced by Co2+, Mg2+, Mn2+ and Ca2+ individually, but is inhibited by Cu2+. Moreover, the activity shows no difference when various sulfur compounds are used as sulfate donors. These results demonstrate
that the ST specific to GTX2/3 is present in the cells of A. tamarense CI01 and is involved in PSP toxin biosynthesis. In addition, the ST from different dinoflagellates is species-specific, which
explains well the various biosynthesis pathways of the PSP toxins in toxic dinoflagellates.
Supported by the National Natural Science Foundation of China (No.40376032) and the Ministry of Science and Technology of
the People’s Republic of China (No.2001CB409700). |
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| Keywords: | Alexandrium tamarense CI01 crude enzyme sulfotransferase paralytic shellfish poisoning |
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