| 螺旋藻多糖的积累与抗肿瘤作用的研究 |
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| 引用本文： | 刘娟妮,王雪青,魏丹,郅琪,李晨韵,庞广昌.螺旋藻多糖的积累与抗肿瘤作用的研究[J].海洋通报,2009,28(6). |
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| 作者姓名： | 刘娟妮 王雪青 魏丹 郅琪 李晨韵 庞广昌 |
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| 作者单位： | 天津市食品生物技术重点实验室,天津,300134;天津商业大学,生物技术与食品科学学院,天津,300134 |
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| 基金项目： | 天津市科委重点基金资助项目(No.08JCZDJC16600);;天津市重大科技攻关项目(No.06YFGZNC04200) |
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| 摘 要： | 在不同温度和光强下培养极大螺旋藻438(Spirulina maxim438),得到其积累多糖规律为:不利于螺旋藻生长的温度、光照条件,会促进胞外多糖EPS(extracellular polysaccharide,EPS)的分泌,而螺旋藻最适生长条件(30℃,5000lx)与胞内多糖IPS(intracellular polysaccharide,IPS)积累的条件一致。MTT(四甲基偶氮唑盐)法体外实验证明IPS和EPS对人宫颈癌Hela细胞有抑制作用,最佳作用浓度分别为80μg/mL,100μg/mL。Caspase-3试剂盒检测经两种多糖作用后Hela细胞Caspase-3酶的变化,结果显示:处理组比对照组Caspase-3酶活性有明显提高,预示着多糖抑制肿瘤细胞的机制可能是通过Caspase途径,引起细胞的凋亡。
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| 关 键 词： | 螺旋藻 培养 多糖 抗肿瘤 Caspase-3 |
Study on accumulation and antitumor effect of polysaccharide from Spirulina maxim |
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| LIU Juan-ni,WANG Xue-qing,WEI Dan,ZHI Qi,LI Chen-yun,PANG Guang-chang.Study on accumulation and antitumor effect of polysaccharide from Spirulina maxim[J].Marine Science Bulletin,2009,28(6). |
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| Authors: | LIU Juan-ni WANG Xue-qing WEI Dan ZHI Qi LI Chen-yun PANG Guang-chang |
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| Institution: | The Key Laboratory of Food and Biotechnology;Tianjin 300134;China;Institute of Biotechnology and Food Science;Tianjin University of Commerce;Tianjin;300134;China |
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| Abstract: | The effects of temperature and illumination on polysaccharides in Spirulina maxima were studied. The results showed that:high temperature and feeble illumination were favorable to the excreting of extracellular polysaccharide. However,the optimum temperature and light for excreting of intracellular polysaccharides were consistent with optimum condition for the growth of Spirulina maxima. Antitumor assay by MIT method indicated that IPS and EPS could inhibit the growth of tumor cells. In particular,the optim... |
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| Keywords: | Spirulina polysaccharides antitumor Caspase-3 |
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