Effects of long-term sample preservation on flow cytometric analysis of natural populations of pico-and nanophytoplankton |
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| Authors: | Mitsuhide Sato Shigenobu Takeda Ken Furuya |
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| Institution: | (1) Graduate School of Agricultural and Life Sciences, The University of Tokyo, Bunkyo-ku, Tokyo 113-8657, Japan |
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| Abstract: | The effects of long-term preservation on flow cytometric parameters of natural oceanic populations of pico-and nanophytoplankton
have been examined. Populations collected at oligotrophic subtropical and subarctic locations in the North Pacific were fixed
with glutaraldehyde and frozen in liquid nitrogen, according to Vaulot et al. (1989). During six months’ storage, chlorophyll red fluorescence declined in all the groups examined, while forward light
scatter was enhanced in Synechococcus and Prochlorococcus, and weakened in nanoeucaryotes. Cell loss was not significant except for Synechococcus. Caution is required when analyzing flow cytometric data of samples stored for more than a month. |
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| Keywords: | Flow cytometry phytoplankton preservation natural community |
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