Phylogenetic relationships within the genus Aspidisca (Protozoa, Ciliophora, Euplotida) revealed by ITS1-5.8S-ITS2 region sequences |
| |
| Authors: | Jie Huang Zhenzhen Yi Miao Miao Khaled A S Al-Rasheid Weibo Song |
| |
| Institution: | (1) Estaci?n Experimental Agropecuaria Rafaela, Instituto Nacional de Tecnolog?a Agropecuaria, C.C. 22, CP 2300 Rafaela, Santa Fe, Argentina;(2) DPMIAC/AFPMB, Walter Reed Army Medical Center, Washington, DC 20307-5001, USA;(3) U.S. National Tick Collection, Institute of Arthropodology and Parasitology, Georgia Southern University, Statesboro, Georgia 30460-8056, USA;(4) Abteilung f?r ?kologie und Parasitologie, Zoologisches Institut I, Kornblumenstrasse 13, 76131 Karlsruhe, Germany;(5) Facultad de Veterinaria, Universidad de Zaragoza, Miguel Servet 177, Zaragoza, CP 50013, Spain;(6) Department of Veterinary Tropical Diseases, Faculty of Veterinary Science, University of Pretoria, Onderstepoort, Pretoria, 0110, South Africa |
| |
| Abstract: | The internal transcribed spacer regions (ITS1 and ITS2) and 5.8S rRNA genes were sequenced in six populations of four Aspidisca species, namely A. leptaspis, A. orthopogon, A. magna and A. aculeata. Phylogenetic trees were constructed by means of Bayesian inference (BI), Maximum Parsimony (MP), Neighbor-Joining (NJ),
and Maximum Likelihood (ML) to assess the inter- and intra-species relationships within the genus Aspidisca. All trees show similar topologies with stable supports and indicate that: (1) four well known groups, i.e., Oligotrichia,
Stichotrichia, Choreotrichia and Hypotrichia, are distinctly outlined within the class Spirotrichea, and all are monophyletic
other than Hypotrichia; (2) members of Aspidisca can be distinguished well, based on the ITS1-5.8S-ITS2 region sequences, and A. leptaspis and A. magna shared a closer relationship than other species; (3) Aspidisca and Euplotes branch early in the subclass Hypotrichia. To compare the phylogenetic relationships based on different genes, SSU rRNA trees
were also constructed with nearly the same species inclusion, which revealed different topologies of inter-species, inter-genera
and inter-subclasses. |
| |
| Keywords: | |
| 本文献已被 万方数据 SpringerLink 等数据库收录！ |
|
