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Cloning and characterization of a delta-6 desaturase encoding gene from Nannochloropsis oculata |
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| Authors: | Xiaolei Ma Jianzhong Yu Baohua Zhu Kehou Pan Jin Pan Guanpin Yang |
| Institution: | MA Xiaolei1,YU Jianzhong1,ZHU Baohua1,PAN Kehou1,**,PAN Jin1,YANG Guanpin2 1Lab of Applied Microalgae Biology,the Key Laboratory of Aquaculture,Ministry of Education,Ocean University of China,Qingdao 266003,China 2College of Marine Life Sciences,China |
| Abstract: | A gene(NANOC-D6D) encoding a desaturase that removes two hydrogen atoms from fatty acids at delta 6 position was isolated from a cDNA library of Nannochloropsis oculata(Droop) D.J.Hibberd(Eustigmatophyceae).The unicellular marine microalga N.oculata synthesizes rich long chain polyunsaturated fatty acids(LCPUFAs),including eicosapentaenoic acid(20:5n-3,EPA).The deduced protein contains 474 amino acids that fold into 4 trans-membrane domains.The neighbor-joining phylogenetic tree indicates that NANOC-D6D is ... |
| Keywords: | microalgae Nannochloropsis oculata EPA delta-6 desaturase gene expression |
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