Influence of acid mine drainage on microbial communities in stream and groundwater samples at Guryong Mine,South Korea |
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| Authors: | Jaisoo Kim So-Yeon Koo Ji-Young Kim Eun-Hee Lee Sang-Don Lee Kyung-Seok Ko Dong-Chan Ko Kyung-Suk Cho |
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| Institution: | (1) Department of Life Science, Kyonggi University, Suwon, 443-760, South Korea;(2) Department of Environmental Science and Engineering, Ewha Womans University, Seoul, 120-750, South Korea;(3) Ground and Geothermal Resources Division, Korea Institute of Geoscience and Mineral Resources, Daejeon, 305-350, South Korea; |
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| Abstract: | The effects of acid mine drainage (AMD) in a stream and groundwater near an abandoned copper mine were characterized by physicochemical
properties, bacterial community structure using denaturing gel gradient electrophoresis (DGGE), and microbial activity/diversity
using Ecoplate technique. Based on DGGE fingerprints, the eubacterial community structures grouped into the stream water (GRS1,
GRS2 and GRS3) and groundwater samples (GW1 and GW2), apparently based on differences in water temperature and the concentrations
of dissolved oxygen, nitrate and sulfate. The most highly AMD-contaminated sample (GRS1) had additional α-Proteobacteria whereas
the groundwater samples included additional β-Proteobacteria, suggesting the development of populations resistant to AMD toxicity
under aerobic and anaerobic conditions, respectively. Community level physiological activities on the 31 Ecoplate substrates
suggested that the activities decreased with increasing concentrations of sulfate and heavy metals derived from AMD. The Shannon
index showed that microbial diversity was greatest in GRS2, and lowest in GRS1, and was probably related to the level of AMD. |
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