The evaluation on biological properties of carboxymethyl-chitosan and carboxymethyl-chitin |
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| Authors: | Jing Chang Wanshun Liu Baoqin Han Bing Liu |
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| Institution: | College of Marine Life Sciences,Ocean University of China,Qingdao 266003,P.R.China |
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| Abstract: | Carboxymethyl-chitosan and carboxymethyl-chitin were prepared with the methods developed in our laboratory.The DS(degree of substitution) and DD (degree of deacetylation) of the carboxymethyl-chitosan were 103.14% and 97.18% respectively,while the DS of the carboxymethyl-chitin was 96.37%.Their effects on human fibroblasts,intradermal irritation test,in vitro and vivodegradability,and biocompatibility were evaluated.The results indicate that the polysaccharides at low concentrations can facilitatethe growth of human fibroblasts and the carboxymethyl-chitosan at 100 μg mL<'-1> is the most effective.The polysaccharides at higherconcentrations,however,inhibit the growth of fibroblasts.The PⅡ (Primary Irritation Index) values of CM-chitosan and CM-chitinare both 0.0,which shows that they have no irritation reaction.Both of the polysaccharides show good degradability and biocompati-bility.Carboxymethyl-chitin degrades faster in vitro than carboxymethyl-chitosan.The latter,however,has no inflammatory reactionafter being implanted in vivo for 7 d and shows better biocompatibility.This study may provide a scientific basis for the use of car-boxymethyl-chitosan and carboxymethyl-chitin as biomaterials. |
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| Keywords: | carboxymethyl-chitosan (CM-chitosan) carboxymethyl-chitin (CM-chitin) biocompatibility biodegradability |
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