| 基于三维荧光光谱-平行因子技术联用的浮游藻化学分类学技术研究 |
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| 引用本文： | 陈小娜,苏荣国,白莹,石晓勇,杨茹君.基于三维荧光光谱-平行因子技术联用的浮游藻化学分类学技术研究[J].海洋学报(英文版),2014,33(12):192-205. |
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| 作者姓名： | 陈小娜 苏荣国 白莹 石晓勇 杨茹君 |
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| 作者单位： | 中国海洋大学化学化工学院, 青岛 266100;中国海洋大学化学化工学院, 青岛 266100;中国海洋大学化学化工学院, 青岛 266100;中国海洋大学化学化工学院, 青岛 266100;中国海洋大学化学化工学院, 青岛 266100 |
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| 基金项目： | The National Natural Science Foundation of China under contract Nos 41376106 and 41276069. |
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| 摘 要： | An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis(PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix(EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis(BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM–PARAFAC–CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios(CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ.
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| 关 键 词： | 荧光激发 因子分析 矩阵 生物类群 发射 平行 优势藻类 特异性识别 |
| 收稿时间： | 2013/9/14 0:00:00 |
| 修稿时间： | 1/9/2014 12:00:00 AM |
Discrimination of marine algal taxonomic groups based on fluorescence excitation emission matrix, parallel factor analysis and CHEMTAX |
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| CHEN Xiaon,SU Rongguo,BAI Ying,SHI Xiaoyong and YANG Rujun.Discrimination of marine algal taxonomic groups based on fluorescence excitation emission matrix, parallel factor analysis and CHEMTAX[J].Acta Oceanologica Sinica,2014,33(12):192-205. |
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| Authors: | CHEN Xiaon SU Rongguo BAI Ying SHI Xiaoyong and YANG Rujun |
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| Institution: | College of Chemistry and Chemical Engineering, Ocean University of China, Qingdao 266100, China |
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| Abstract: | An in vivo three-dimensional fluorescence method for the determination of algae community structure was developed by parallel factor analysis (PARAFAC) and CHEMTAX. The PARAFAC model was applied to fluorescence excitation-emission matrix (EEM) of 60 algae species belonging to five divisions and 11 fluorescent components were identified according to the residual sum of squares and specificity of the composition profiles of fluorescent. By the 11 fluorescent components, the algae species at different growth stages were classified correctly at the division level using Bayesian discriminant analysis (BDA). Then the reference fluorescent component ratio matrix was constructed for CHEMTAX, and the EEM-PARAFAC-CHEMTAX method was developed to differentiate algae taxonomic groups. The correct discrimination ratios (CDRs) when the fluorometric method was used for single-species samples were 100% at the division level, except for Bacillariophyta with a CDR of 95.6%. The CDRs for the mixtures were above 94.0% for the dominant algae species and above 87.0% for the subdominant algae species. However, the CDRs of the subdominant algae species were too low to be unreliable when the relative abundance estimated was less than 15.0%. The fluorometric method was tested using the samples from the Jiaozhou Bay and the mesocosm experiments in the Xiaomai Island Bay in August 2007. The discrimination results of the dominant algae groups agreed with microscopy cell counts, as well as the subdominant algae groups of which the estimated relative abundance was above 15.0%. This technique would be of great aid when low-cost and rapid analysis is needed for samples in a large batch. The fluorometric technique has the ability to correctly identify dominant species with proper abundance both in vivo and in situ. |
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| Keywords: | CHEMTAX fluorescence excitation-emission matrices in vivo fluorescent components parallel factor analysis phytoplankton |
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