| 泥鳅(Misgurnus anguillicaudatus)病原霍乱弧菌(Vibrio cholerae)的表型与分子鉴定 |
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| 引用本文： | 邴旭文,阎斌伦,张晓君,秦 蕾,毕可然.泥鳅(Misgurnus anguillicaudatus)病原霍乱弧菌(Vibrio cholerae)的表型与分子鉴定[J].海洋与湖沼,2009,40(6):692-698. |
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| 作者姓名： | 邴旭文 阎斌伦 张晓君 秦 蕾 毕可然 |
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| 作者单位： | 1. 中国水产科学研究院淡水渔业研究中心,农业部淡水鱼类遗传育种和养殖生物学重点开放实验室,无锡,214081
2. 淮海工学院海洋学院,江苏省海洋生物技术重点建设实验室,连云港,222005 |
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| 基金项目： | 农业部淡水鱼类遗传育种和养殖生物学重点开放实验室开放基金资助,BZ2009-03号;;连云港市科技攻关项目资助,CN0826号 |
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| 摘 要： | 从江苏连云港某养殖场养殖死亡的泥鳅肝脏、血液及腹水中分离出大量优势生长的细菌,人工感染试验证明其对泥鳅具有很强的致病性。采用表观分类学及分子生物学方法,对分离菌进行了形态特征、理化特性、胞外酶活性及溶血活性等生物学性状检验;用PCR方法同时扩增其16SrRNA和gyrB基因,分析了16SrRNA和gyrB两种基因序列的同源性,并构建了系统发生树,通过基因序列分析,比较了两种基因在相似细菌的检测和鉴定能力;基于16SrRNA和gyrB基因的系统发育学分析表明分离菌(LD081008B-1)所扩增的16SrRNA和gyrB基因序列均与GenBank数据库中霍乱弧菌具有较高的相似性,且gyrB基因用于细菌种间鉴定更具优越性;16SrRNA基因序列长度为1446bp(GenBank登录号:GQ205447),gyrB基因序列长度为1207bp(GenBank登录号:GQ205452);根据分离菌的表型特征及分子特征,判定病原菌为弧菌属(VibrioPacini1854)的霍乱弧菌(Vibriocholerae)。胞外酶活性及溶血活性检测表明分离菌均具有蛋白酶、卵磷脂酶、淀粉酶、明胶酶及DNA酶活性,在含7%家兔脱纤血...
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| 关 键 词： | 泥鳅 霍乱弧菌 生物学特性 16SrRNA基因 gyrB基因 |
| 收稿时间： | 2008/12/15 0:00:00 |
| 修稿时间： | 2009/2/23 0:00:00 |
PHENOTYPIC AND MOLECULAR IDENTIFICATION OF PATHOGENIC VIBRIO CHOLERAE ISOLATED FROM MISGURNUS ANGUILLICAUDATUS |
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| BING Xu-Wen,YAN Bin-Lun,ZHANG Xiao-Jun,QIN Lei and BI Ke-Ran.PHENOTYPIC AND MOLECULAR IDENTIFICATION OF PATHOGENIC VIBRIO CHOLERAE ISOLATED FROM MISGURNUS ANGUILLICAUDATUS[J].Oceanologia Et Limnologia Sinica,2009,40(6):692-698. |
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| Authors: | BING Xu-Wen YAN Bin-Lun ZHANG Xiao-Jun QIN Lei and BI Ke-Ran |
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| Institution: | 1. Key Laboratory of Genetic Breeding and Aquaculture Biology of Freshwater Fishes;Ministry of Agriculture;Freshwater Fisheries Research Center;Chinese Academy of Fishery Science;Wuxi;214081;2. College of Ocean;Key Laboratory of Oceanic Biotechnology of Jiangsu;Huaihai Institute of Technology;Lianyungang;222005 |
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| Abstract: | A serious mortality of cultured loach Misgurnus anguillicaudatus occurred in some farms in Donghai County, Jiangsu Province in October 2008. Syndrome included bleeding in head, opercula, lower jaw, fins, and fin bases, swollen muscle, anus, liver, and spleen, and empty intestines. Tissues were sampled from liver, bleed, and ascitic fluid of diseased fish, from which the pathogen were isolated, and the biological characteristics were examined, including characteristics in morphology, physiology, and biochemi... |
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| Keywords: | Misgurnus anguillicaudatus L Vibrio cholerae Biological characteristics 16S rRNA gene gyrB gene |
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